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Comparison of Chloroflexus aurantiacus strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically

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Abstract

Chloroflexus aurantiacus J-10-fl is a thermophilic green bacterium, a filamentous anoxygenic phototroph, and the model organism of the phylum Chloroflexi. We applied high-throughput, liquid chromatography–mass spectrometry in a global quantitative proteomics investigation of C. aurantiacus cells grown under oxic (chemoorganoheterotrophically) and anoxic (photoorganoheterotrophically) redox states. Our global analysis identified 13,524 high-confidence peptides that matched to 1,286 annotated proteins, 242 of which were either uniquely identified or significantly increased in abundance under photoheterotrophic culture condition. Fifty-four of the 242 proteins are previously characterized photosynthesis-related proteins, including chlorosome proteins, proteins involved in the bacteriochlorophyll biosynthesis, 3-hydroxypropionate (3-OHP) CO2 fixation pathway, and components of electron transport chains. The remaining 188 proteins have not previously been reported. Of these, five proteins were found to be encoded by genes from a novel operon and observed only in photoheterotrophically grown cells. These proteins candidates may prove useful in further deciphering the phototrophic physiology of C. aurantiacus and other filamentous anoxygenic phototrophs.

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Abbreviations

FAP:

Filamentous anoxygenic phototrophic

3-OHP:

3-Hydroxypropionate

BChl:

Bacteriochlorophyll

ACIII:

Alternative complex III

LC–MS:

Liquid chromatography–mass spectrometry

HPLC:

High pressure liquid chromatography

COG:

Clusters of orthologous groups

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Acknowledgments

The research described in this article was funded by the Genomic Science Program sponsored by the U. S. Department of Energy Office of Biological and Environmental Research (DOE/BER) and performed in the Environmental Molecular Sciences Laboratory, a DOE/BER national scientific user facility located at Pacific Northwest National Laboratory (PNNL) in Richland, Washington. PNNL is a multi-program national laboratory operated by Battelle for the DOE under Contract DE-ACO5-76RLO 1830. D. A. B. additionally acknowledges support from DOE Office of Basic Energy Sciences (DE-FG02-94ER20137). The authors wish to acknowledge Dr. Yusuke Tsukatani for helpful discussion. Mass spectrometry data used in this study can be requested at http://omics.pnl.gov.

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Authors and Affiliations

  1. Biological Separations and Mass Spectrometry, Pacific Northwest National Laboratory, Richland, WA, 99352, USA

    Li Cao, Athena A. Schepmoes, Richard D. Smith, Mary S. Lipton & Stephen J. Callister

  2. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 16802, USA

    Donald A. Bryant & Kajetan Vogl

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  1. Li Cao
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  2. Donald A. Bryant
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  7. Stephen J. Callister
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Correspondence to Stephen J. Callister.

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This manuscript has been authored by Battelle Memorial Institute under Contract No. DE-AC05-76RL01830 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes.

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Cao, L., Bryant, D.A., Schepmoes, A.A. et al. Comparison of Chloroflexus aurantiacus strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically. Photosynth Res 110, 153–168 (2012). https://doi.org/10.1007/s11120-011-9711-8

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