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A diversity of resistance sources to Fusarium oxysporum f. sp. pisi found within grass pea germplasm

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Abstract

Purpose

Grass pea is a legume species with recognized resistance to several diseases and thus important for the improvement of related major legume crops. It is infected by the soil-borne fungus Fusarium oxysporum f. sp. pisi (Fop), known as causal agent of pea fusarium wilt. We aim to identify, among grass pea, new sources of resistance against Fop and characterize the detected resistance mechanisms.

Methods

A grass pea worldwide collection of accessions was characterized under controlled conditions for response to Fop race 2. Fungal colonization dynamics and potential resistance mechanisms were studied through confocal laser scanning microscopy (CLSM) using Fop race 2 expressing green fluorescent protein (GFP).

Results

A quantitative nature of resistance to Fop, ranging from highly to partially resistant and susceptible accessions was detected, with resistance being the most frequent phenotype. Diverse colonization patterns were observed, suggesting the existence of different resistance mechanisms. In the highly resistant accessions, absence of fungal colonization in the vascular tissue was detected, while fungal progression was arrested at the level of roots both in highly resistant and partially resistant accessions.

Conclusions

The resistant accessions identified here can be exploited in grass pea breeding for fusarium wilt resistance, and due to the phylogenetic relatedness to pea, potentially contribute to pea improvement.

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Data availability

The datasets used during the current study are available from the corresponding author on reasonable request.

References

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Acknowledgements

The authors are thankful to Priscila Pereira (ITQB NOVA) for grass pea seed multiplication, Carmen Santos (ITQB NOVA) for collaboration in fungal inoculation, Esther Aguilera and Tânia Fernandes (Universidad de Córdoba) for the help with the Fop-GFP protoplast transformation and Mariana Ferreira (ITQB NOVA) for the confocal laser scanning microscopy images acquisition.

Funding

Financial support by Fundação para a Ciência e Tecnologia (FCT), Portugal, is acknowledge through grant PD/BD/114418/2016 (AMS), the research unit GREEN-IT (UID/04551/2020), the QuaLaty project (PTDC/AGR-TEC/0992/2014), and the Spanish Research Agency (AEI) projects AGL2017-82907-R and BIO2016-78923-R. We also thank to the FP7 EU funding through the LEGATO project (grant agreement n°FP7-613551). This work was partially supported by PPBI- Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE – “Orçamento de Estado” and by European funds from FEDER - “Fundo Europeu de Desenvolvimento Regional”.

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Authors and Affiliations

Authors

Contributions

Ana Margarida Sampaio performed fungal inoculations, disease assessment, data processing and analysis, Fop-GFP transformation, confocal laser scanning microscopy images acquisition and drafted the manuscript. Stefania Vitale and David Turrà collaborated in Fop-GFP transformation and revised the manuscript. Antonio Di Pietro coordinated the Fop-GFP transformation and revised the manuscript. Diego Rubiales participated in results discussion and revised the manuscript. Fred van Eeuwijk participated in the phenotyping data analysis and revised the manuscript. Maria Carlota Vaz Patto coordinated the study, participated in results discussion and in drafting and manuscript revision.

Corresponding author

Correspondence to Ana Margarida Sampaio.

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The authors declare no competing interests.

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Responsible Editor: Stéphane Compant.

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Sampaio, A.M., Vitale, S., Turrà, D. et al. A diversity of resistance sources to Fusarium oxysporum f. sp. pisi found within grass pea germplasm. Plant Soil 463, 19–38 (2021). https://doi.org/10.1007/s11104-021-04895-z

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  • DOI: https://doi.org/10.1007/s11104-021-04895-z

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