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Screening of rhizosphere bacteria from grapevine for their suppressive effect on Xiphinema index Thorne & Allen on in vitro grape plants

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Abstract

Xiphinema index is the most aggressive root parasite affecting vines in Chile, controlled mainly chemically. The aim of this study was to identify rhizobacteria in grapevine roots growing in infested soils and to determine if some of these affect the parasitism caused by the nematode. Rhizobacteria of ungrafted grapevine cultivars from 11 vineyards were isolated using TSBA and identified with FAMEs analysis. The antagonistic effect to X. index of those from soils 1 to 4 was assessed in 500 ml pots treating 2 months old in vitro plants with a bacterial suspension containing 1 × 106 c.f.u./ml and 400 nematodes, determining after 4 months growth nematode populations and root damages. Also culture filtrates were assessed. 400 isolates in 25 genera were obtained from the 11 vineyards. The most frequent species were Pseudomonas putida (35.1%), Escherichia coli (7.6%) and Pseudomonas fluorescens (6.1%). Isolates of soils 1 to 4, show that Bacillus megaterium, B. brevis, Pseudomonas corrugata, P. savastanoi, Stenotrophomonas maltophilia and Serratia plymuthica reduced root damage and suppressed populations, meanwhile strains of B. brevis and Comamonas acidovorans increased plant growth but did not control nematodes. It is concluded that these isolates may be useful in biological control programmes in vineyards.

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Abbreviations

GFLV:

Grape fan leaf virus

TSBA:

Tryptic soy broth agar

FAMEs:

Fatty acids methyl esters

MIS:

Microbial identification system

RDA:

Redundancy analysis

ANOVA:

Analysis of variance

SIs:

Similarity indices

PGPR:

Plant growth promoting rhizobacteria

ACC:

1-Aminocyclopropane-1-carboxylate

References

  • Aballay E, Benavides F, Vieira A (1998) Resistencia de algunos portainjertos a una población chilena de Xiphinema index. Nematol Med 26(2):185–188

    Google Scholar 

  • Aballay E, Persson P, Mårtensson A (2009) Plant-parasitic nematodes in Chilean vineyards. Nematropica 39(1):85–98

    Google Scholar 

  • Ait Barka E, Belarbi A, Hachet C, Nowak J, Audran J (2000) Enhancement of in vitro growth and resistance to gray mould of Vitis vinifera co-cultured with plant growth-promoting rhizobacteria. FEMS Microbiol Lett 186:91–95

    Article  Google Scholar 

  • Ali NI, Siddiqui IA, Shaukat SS, Zaki MJ (2002) Nematicidal activity of some isolates of Pseudomonas spp. Soil Biol Biochem 34:1051–1058

    Article  CAS  Google Scholar 

  • Bell CR, Dickie GA, Harvey WLG, Chan JWYF (1995) Endophytic bacteria in grapevine. Can J Microbiol 41(1):46–53

    Article  CAS  Google Scholar 

  • Berg G, Eberl L, Hartmann A (2005) The rhizosphere as a reservoir for opportunistic human pathogenic bacteria. Environ Microbiol 7(11):1673–1685

    Article  PubMed  CAS  Google Scholar 

  • Braun-Kiewnick A, Sands DC (2001) Gram-negative bacteria, Pseudomonas. In: Schaad NE, Jones JB, Chun W (eds) Laboratory guide for identification of plant pathogenic bacteria, 3rd edn. APS Press, Minnesota, pp 84–120

    Google Scholar 

  • Brown DJ, Boag B (1988) An examination of methods used to extract virus-vector nematodes (nematoda: longidoridae and trichodoridae) from soil samples. Nematol Mediterr 16:93–99

    Google Scholar 

  • Chee R (1980) The effects of growth substances and photoperiod on shoot apices of Vitis cultured in vitro, and their effects on subcultured shoots tips. M.S Thesis, Cornell University, Ithaca, New York, USA

  • Compant S, Reiter B, Sessitsch A, Nowak J, Clément C, Ait Barka E (2005) Endophytic colonization of Vitis vinífera L. by plant growth-promoting bacterium Burkholderia sp. isolate PsJN. Appl Environ Microbiol 71(4):1685–1693

    Article  PubMed  CAS  Google Scholar 

  • Coplin DL, Kado CI (2001) Gram-negative bacteria, Pantoea. In: Schaad NE, Jones JB, Chun W (eds) Laboratory guide for identification of plant pathogenic bacteria, 3rd edn. APS Press, Minnesota, pp 73–83

    Google Scholar 

  • Dong LQ, Zhang KQ (2006) Microbial control of plant-parasitic nematodes: a five-party interaction. Plant Soil 288:31–45

    Article  CAS  Google Scholar 

  • Farag N, Stead DE, Janse DJ (1999) Ralstonia (Pseudomonas) solanacearum race 2, biovar 1, detected in surface (irrigation) water in Egypt. J Phytopathol 147:485–487

    Article  Google Scholar 

  • Fiore N, Prodan S, Montealegre J, Aballay E, Pino A, Zamorano A (2008) Survey of grapevine viruses in Chile. J Plant Pathol 90(1):125–130

    CAS  Google Scholar 

  • Germida JJ, Siciliano SD, de Freitas JR, Seib AM (1998) Diversity of root-associated bacteria associated with field-grown canola (Brassica napus L.) and wheat (Triticum aestivum L.). FEMS Microbiol Ecol 26:43–50

    Article  CAS  Google Scholar 

  • Glick BR, Penrose DM, Li J (1998) A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. J Theor Biol 190:63–68

    Article  PubMed  CAS  Google Scholar 

  • González H (2007) Nemátodos fitoparásitos que afectan a frutales y vides en Chile. Inst Investig Agrop INIA, Santiago

    Google Scholar 

  • Gu YQ, Mo HM, Zhou JP, Zou CS, Zhang KQ (2007) Evaluation and identification of potential organic nematicidal volatiles from soil bacteria. Soil Biol Biochem 39:2567–2575

    Article  CAS  Google Scholar 

  • Huettel RN, Rebois RV (1985) Culturing plant parasitic nematodes using root explants. In: Zuckerman BM, Mai WF, Harrison MB (eds) Plant nematology laboratory manual. The University of Massachusetts Agricultural Experiment Station, Amherst, pp 155–158

    Google Scholar 

  • Insunza V, Alstrom S, Eriksson KB (2002) Root bacteria from nematicidal plants and their biocontrol potential against trichodorid nematodes in potato. Plant Soil 241:271–278

    Article  CAS  Google Scholar 

  • Johansson PM, Johnson L, Gerhardson B (2003) Suppression of wheat-seedling disease caused by Fusarium culmorum and Micordium nivale, using bacterial seed treatment. Plant Pathol 52:219–227

    Article  Google Scholar 

  • Kerry BR (2000) Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes. Annu Rev Phytopathol 38:423–441

    Article  PubMed  CAS  Google Scholar 

  • Kloepper JW, Beauchamp C (1992) A review of issues related to measuring colonization of plant roots by bacteria. Can J Microbiol 38:1219–1232

    Article  Google Scholar 

  • Kloepper JW, Rodriguez-Kabana R, McInroy J, Young RW (1992) Rhizosphere bacteria antagonistic to soybean cyst (Heterodera glycines) and root-knot (Meloidogyne incognita) nematodes: identification by fatty acid analysis and frequency of biological control activity. Plant Soil 139:75–84

    Article  CAS  Google Scholar 

  • Kloepper JW, Rodriguez-Kábana R, McInroy JA, Collins DJ (1991) Analysis of populations and physiological characterization of microorganisms in rhizospheres of plants with antagonistic properties to phytopathogenic nematodes. Plant Soil 136:95–102

    Article  Google Scholar 

  • Kloepper JW, Rodríguez-Kábana R, Zehnder GW, Murphy JE, Sikora E, Fernandez C (1999) Plant root-bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases. Australas Plant Pathol 28:21–26

    Article  Google Scholar 

  • Kluepfel DA, McInnis TM, Zehr EI (1993) Involvement of root-colonizing bacteria in peach orchard soils suppressive of the nematode Criconemella xenoplax. Phytopathology 83(11):1240–1245

    Article  Google Scholar 

  • Kose C, Guleryuz M, Sahn F, Demrts I (2003) Effects of some plant growth promoting rhizobacteria (PGPR) on rooting of grapevine rootstocks. Acta Agrobot 56:47–52

    Google Scholar 

  • Leps L, Smilauer P (1999) Multivariate analysis of ecological data. Faculty of Biological Sciences, University of of South Bohemia Ceské Budejovice

  • Lian LH, Tian BY, Xiong R, Zhu MZ, Xu J, Zhang KQ (2007) Proteases from Bacillus: a new insight into the mechanism of action for rhizobacterial suppression of nematode populations. Lett Appl Microbiol 45:262–269

    Article  PubMed  CAS  Google Scholar 

  • Lodewyckx C, Vangronsveld J, Porteus F, Moore ERB, Taghavi S, Mezgeay M, Van der Lelie D (2002) Endophytic bacteria and their potential applications. Critic Rev Plant Sci 21(6):583–606

    Article  Google Scholar 

  • Mendoza AR, Kiewnick S, Sikora R (2008) In Vitro activity of Bacillus firmus against the burrowing nematode Radopholus similis, the root-knot nematodo Meloidogyne incognita and the stem nematode Ditylenchus dipsaci. Bio Sci Tech 18(4):377–389

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised media for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Oliveira DF, Campos VP, Amaral DR, Nunes AS, Pantaleao JA, Costa DA (2007) Selection of rhizobacteria able to produce metabolites active against Meloidogyne exigua. Eur J Plant Pathol 119:477–479

    Article  CAS  Google Scholar 

  • Sasser M (1990) Identification of bacteria through fatty acid analysis. In: Methods in phytobacteriology. Akademiai Kiado, Budapest (HU): 199–204

  • Scortichini M, Rossi MP, Loreti S, Bosco A, Fiori M, Jackson RW, Otead DE, Aspin A, Marchesi U, Zini M, Janse JD (2005) Pseudomonas syringae pv. coryli, the causal agent of bacterial twig dieback of Corylus avellana. Phytopathol 95:136–1324

    Article  Google Scholar 

  • Siddiqui IA, Hass D, Heeb S (2005) Extracellular protease of pseudomonas fluorescens CHAO, a biocontrol factor with activity against the root-knot nematode Meloidogyne incognita. Appl Environ Microbiol 71:5646–5649

    Article  PubMed  CAS  Google Scholar 

  • Siddiqui ZA, Baghel G, Akhtar MS (2007) Biocontrol of Meloidogyne javanica by Rhizobium and plant growth-promoting rhizobacteria on lentil. World J Microbiol Biotechnol 23:435–441

    Article  CAS  Google Scholar 

  • Siddiqui ZA, Mahmood I (1999) Role of bacteria in the management of plant parasitic nematodes: a review. Bioresour Technol 69:167–179

    Article  CAS  Google Scholar 

  • Sikora R (1997) Biological system management in the rhizosphere an inside-out/outside-in perspective. Med Fac Landbouww Univ Gent 62/2a: 105–112

  • Tash K (2005) Rahnella aquatilis bacteremia from a suspected urinary source. J Clin Microbiol 43(5):2526–2528

    Article  PubMed  Google Scholar 

  • Tian B, Yang J, Zhang KQ (2007) Bacteria used in the biological control of plant-parasitic nematodes: populations, mechanisms of action and future prospects. FEMS Microbiol Ecol 61:197–213

    Article  PubMed  CAS  Google Scholar 

  • Valenzuela A, Aballay E (1996) Evaluación del control químico de Xiphinema index en vides. Nematropica 26:177–179

    Google Scholar 

  • Valenzuela A, Aballay E, Torres M (1992) Identificación y frecuencia de nemátodos asociados a la vid en la Región Metropolitana, Chile. Investig Agric (Chile) 12:15–17

    Google Scholar 

  • Weller D, Raaijmakers JM, McSpadden BB, Thomashow LS (2002) Microbial populations responsible for specific soil suppressiveness to plant pathogens. Annu Rev Phytopathol 40:309–348

    Article  PubMed  CAS  Google Scholar 

  • Weller SA, Aspin A, Stead DE (2000) Classification and identification of plant-associated bacteria by fatty acid profiling. Bull OEPP/EPPO Bull 30:375–380

    Google Scholar 

  • West ER, Cother EJ, Steel CC, Ash GJ (2010) The characterization and diversity of bacterial endophytes of grapevine. Can J Microbiol 56:209–216

    Article  PubMed  CAS  Google Scholar 

  • Westphal A (2005) Detection and description of soils with specific nematode suppressiveness. J Nematol 37:121–130

    PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. Nicola Greco, from Istituto per la Protezione Delle Piante, C.N.R, Bari, Italy, for critically reviewing the manuscript.

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Authors and Affiliations

  1. Department of Crop Protection, Faculty of Agronomical Sciences, University of Chile, P.O. Box 1004, Santiago, Chile

    Erwin Aballay

  2. Department of Soil and Environment, Swedish University of Agricultural Sciences, Box 7014, SE- 750 07, Uppsala, Sweden

    Anna Mårtensson

  3. Department of Crop Production Ecology, Swedish University of Agricultural Sciences, Box 7043, SE- 750 07, Uppsala, Sweden

    Paula Persson

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  1. Erwin Aballay
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  2. Anna Mårtensson
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  3. Paula Persson
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Correspondence to Erwin Aballay.

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Responsible Editor: Jorge Vivanco.

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Aballay, E., Mårtensson, A. & Persson, P. Screening of rhizosphere bacteria from grapevine for their suppressive effect on Xiphinema index Thorne & Allen on in vitro grape plants. Plant Soil 347, 313–325 (2011). https://doi.org/10.1007/s11104-011-0851-6

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