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Overexpression of vacuolar transporters OsVIT1 and OsVIT2 reduces cadmium accumulation in rice

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Abstract

Excessive cadmium in rice grain in agricultural production is an important issue to be addressed in some southern regions of China. In this study, we constructed transgenic rice overexpressing OsVIT1 and OsVIT2 driven by 35S promoter in the cultivar ZH11. Compared with ZH11, OsVIT1 expression in leaves was significantly increased by 3–6.6 times and OsVIT2 expression in leaves was significantly increased by 2–2.5 times. Hydroponic experiments showed that overexpression of OsVIT1 and OsVIT2 increased the tolerance to Fe deficiency, significantly reduced Cd content in shoot and xylem sap, and had no effect on Cd tolerance in rice. Two years of field trials showed that the Fe content in the grain of OsVIT1 and OsVIT2 overexpressed materials was significantly reduced by 20–40% and the straw Fe content was significantly increased by 10–45%, and the grain Fe content distribution ratio was significantly decreased and the straw Fe distribution ratio was significantly increased compared with the wild type. The OsVIT1 and OsVIT2 overexpressed materials significantly reduced the Cd content of grain by 40–80% and the Cd content of straws by 37–77%, and the bioconcentration factor of Cd was significantly reduced in both grains and straw of OsVIT1 and OsVIT2 overexpressed materials. Overexpression of OsVIT1 and OsVIT2 did not affect the concentration of other metal ions in rice straw and grain. qRT-PCR analysis showed that the expression of the low affinity cation transporter OsLCT1 was significantly downregulated in the OsVIT1 and OsVIT2 overexpressed materials. In conclusion, overexpression of OsVIT1 and OsVIT2 reduced Cd accumulation in straw and grains, providing a strategy for Cd reduction in rice.

Key message

Overexpression of vacuolar transporter OsVIT1 and OsVIT2 reduced Cd accumulation in straw and grains, providing a strategy for Cd reduction in rice

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Funding

This research was funded by the National Key Research and Development Program of China (2022YFD1700103), the Natural Science Foundation of Hunan Province, China (2021RC3086, 2021JC0001-05), the China Agriculture Research System Project (CARS-01-02A), and the National Natural Science Foundation of China (31800202).

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Authors and Affiliations

  1. College of Resources, Hunan Agricultural University, Changsha, 410128, China

    Jin-Song Luo, Jing Tang, Yiqi He, Dong Liu, Yilin Yang & Zhenhua Zhang

  2. Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Provincial Key Laboratory of Nutrition in Common University, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Changsha, 410128, China

    Jin-Song Luo, Jing Tang, Yiqi He, Dong Liu, Yilin Yang & Zhenhua Zhang

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  1. Jin-Song Luo
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  2. Jing Tang
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  3. Yiqi He
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  4. Dong Liu
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  5. Yilin Yang
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  6. Zhenhua Zhang
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Contributions

Jin-Song Luo and Zhenhua Zhang designed the experiments; Yiqi He performed most of the experiments; Dong Liu and Yilin Yang performed part of the experiments; Jin-Song Luo and Yiqi He analyzed the data; and Jing Tang and Jin-Song Luo wrote the manuscript.

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Correspondence to Jin-Song Luo or Zhenhua Zhang.

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Luo, JS., Tang, J., He, Y. et al. Overexpression of vacuolar transporters OsVIT1 and OsVIT2 reduces cadmium accumulation in rice. Plant Mol Biol 114, 14 (2024). https://doi.org/10.1007/s11103-023-01405-w

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