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The zinc-finger transcription factor ZAT6 is essential for hydrogen peroxide induction of anthocyanin synthesis in Arabidopsis

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Abstract

The accumulation of flavonoids is activated by various abiotic stresses, and the induction of reactive oxygen species (ROS) especially hydrogen peroxide (H2O2) is a general response to abiotic stress in plants. However, the direct link between flavonoids and H2O2 and underlying mechanism remain elusive. In this study, we found that the concentrations of anthocyanin and flavonoids were significantly induced by H2O2 treatment. Furthermore, we found that the transcript level of ZINC FINGER of ARABIDOPSIS THALIANA 6 (ZAT6) was significantly activated after exogenous H2O2 treatment, and modulation of AtZAT6 expression positively affected the concentrations of both anthocyanin and total flavonoids. Notably, exogenous H2O2-induced anthocyanin synthesis was largely alleviated in AtZAT6 knockdown plants, but showed higher level in AtZAT6 overexpressing plants. AtZAT6 directly activated the expressions of TT5, TT7, TT3, TT18, MYB12, and MYB111 through binding to their promoters with TACAAT elements of these genes, and the activation of MYB12 and MYB111 up-regulated the expressions of TT4 and TT6. Taken together, this study indicates that AtZAT6 plays important role in H2O2-activated anthocyanin synthesis, via directly binding to the promoters of several genes that involved in anthocyanin synthesis.

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Acknowledgements

This research was supported by National Natural Science Foundation of China (No. 31570249), the startup funding and the Scientific Research Foundation of Hainan University (No. kyqd1531) to Haitao Shi.

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Authors and Affiliations

  1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China

    Haitao Shi, Guoyin Liu & Yunxie Wei

  2. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

    Zhulong Chan

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  1. Haitao Shi
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  2. Guoyin Liu
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  3. Yunxie Wei
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  4. Zhulong Chan
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HS conceived and designed the experiments. HS, GL and YW performed the experiments and analyzed the manuscript. HS wrote and revised the manuscript. ZC provided suggestions and revised the manuscript.

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Correspondence to Haitao Shi or Zhulong Chan.

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Shi, H., Liu, G., Wei, Y. et al. The zinc-finger transcription factor ZAT6 is essential for hydrogen peroxide induction of anthocyanin synthesis in Arabidopsis. Plant Mol Biol 97, 165–176 (2018). https://doi.org/10.1007/s11103-018-0730-0

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