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Arabidopsis drought-induced protein Di19-3 participates in plant response to drought and high salinity stresses

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Abstract

Di19 (drought-induced protein19) family is a novel type of Cys2/His2 zinc-finger proteins. In this study, Arabidopsis Di19-3 was functionally characterized. The experimental results revealed that AtDi19-3 is a transcriptional activator, and could bind to the TACA(A/G)T sequence. AtDi19-3 expression in plants was remarkably induced by NaCl, mannitol and abscisic acid (ABA). T-DNA insertion mutation of AtDi19-3 results in an increase in plant tolerance to drought and high salinity stresses and ABA, whereas overexpression of AtDi19-3 leads to a drought-, salt- and ABA-sensitive phenotype of the transgenic plants. In the presence of NaCl, mannitol or ABA, rates of seed germination and cotyledon greening in Atdi19-3 mutant were higher, but in AtDi19-3 overexpression transgenic plants were lower than those in wild type. Roots of Atdi19-3 mutant seedlings were longer, but those of AtDi19-3 overexpression transgenic seedlings were shorter than those of wild type. Chlorophyll and proline contents in Atdi19-3 mutant were higher, but in AtDi19-3 overexpression seedlings were lower than those in wild type. Atdi19-3 mutant showed greater drought-tolerance, whereas AtDi19-3 overexpression transgenic plants exhibited more drought-sensitivity than wild type. Furthermore, expression of the genes related to ABA signaling pathway was altered in Atdi19-3 mutant and AtDi19-3 transgenic plants. These data suggest that AtDi19-3 may participate in plant response to drought and salt stresses in an ABA-dependent manner.

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Acknowledgments

Authors thank Professor Jeff Harper in University of Nevada (Reno, NV89557, USA) for kindly providing Atdi19-3 T-DNA insertion mutant seeds. This work was supported by the project from the Ministry of Agriculture of China for Transgenic Research (Grant No. 2014ZX08009-27B) and Natural Sciences Foundation of Hubei Province (Grant No. 2012FFA126, 2013CFA119).

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  1. Li-Xia Qin

    Present address: Institute of Cotton, Shanxi Academy of Agricultural Sciences, Yuncheng, 044000, China

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  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China

    Li-Xia Qin, Yang Li, Deng-Di Li, Wen-Liang Xu, Yong Zheng & Xue-Bao Li

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Qin, LX., Li, Y., Li, DD. et al. Arabidopsis drought-induced protein Di19-3 participates in plant response to drought and high salinity stresses. Plant Mol Biol 86, 609–625 (2014). https://doi.org/10.1007/s11103-014-0251-4

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