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Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1

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Abstract

The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA processing. We found EYFP-labeled CBF5 of A. thaliana to be located within nucleoli and Cajal bodies, but neither at centromeres nor somewhere else on chromosomes. Arabidopsis mutants carrying a homozygous T-DNA insertion at the CBF5 locus were lethal. Yeast two-hybrid and mRNA expression analyses demonstrated that AtCBF5 is co-expressed and interacts with a previously uncharacterized protein containing a conserved NAF1 domain, presumably involved in H/ACA box snoRNP biogenesis. The homologous yeast protein has been shown to contribute to RNA pseudouridinylation. Thus, AtCBF5 might have an essential function in RNA processing rather than being a kinetochore protein.

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Abbreviations

3AT:

3-amino-triazole

BiFC:

Bimolecular fluorescence complementation

CB:

Cajal bodies

CDD:

Conserved domain database

DAPI:

4′,6-diamidino-2-phenylindole

DIC:

Differential interference contrast

PPT:

Phosphinotricine

snRNAs:

Small nuclear RNAs

snRNPs:

Small nuclear ribonucleoproteins

snoRNAs:

Small nucleolar RNAs

snoRNPs:

Small nucleolar ribonucleoproteins

TMG-capped-snRNA:

tri-methylguanosine-capped snRNA

Ψ:

Pseudouridine

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Acknowledgements

We thank Andrea Kunze and Rita Schubert for technical assistance, Bernhard Claus for help with confocal microscopy, Jörg Fuchs for flow-sorting of nuclei, Alice Navratilova for help with examination of field bean hairy root cultures, Sabina Klatte and Alexander Kukalev for helpful discussions, Karin Lipfert and Ursula Tiemann for help with preparation of figures. This work was supported by a grant from the Deutsche Forschungsgemeinschaft to I.S. (Schu 951/9-3).

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Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany

    Inna Lermontova, Veit Schubert & Ingo Schubert

  2. Department of Biochemistry, Friedrich-Alexander University Erlangen-Nürnberg, Straudtstrasse 5, 91058, Erlangen, Germany

    Frederik Börnke

  3. Institute of Plant Molecular Biology, Branisovska 31, 37005, Ceske Budejovice, Czech Republic

    Jiri Macas

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  1. Inna Lermontova
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Correspondence to Inna Lermontova.

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Lermontova, I., Schubert, V., Börnke, F. et al. Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1. Plant Mol Biol 65, 615–626 (2007). https://doi.org/10.1007/s11103-007-9226-z

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  • DOI: https://doi.org/10.1007/s11103-007-9226-z

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