Photosynthetica 2014, 52(4):501-510 | DOI: 10.1007/s11099-014-0058-1

Effect of selenium on CO2 and NO3- assimilation under low and adequate nitrogen supply in wheat (Triticum aestivum L.)

R. Hajiboland1,2,*, N. Sadeghzade2
1 Center of Excellence for Biodiversity, University of Tabriz, Tabriz, Iran
2 Plant Science Department, University of Tabriz, Tabriz, Iran

In order to study the mechanisms of Se-mediated growth improvement as related to carbon (C) and nitrogen (N) metabolism, wheat plants were cultivated hydroponically with adequate (4 mM, Na) or low (1 mM, Nd) N supply and treated with 10 and 50 μM Na2SeO4 for six weeks. The Se supplementation enhanced plant biomass; it was significant for shoots of Na plants at 50 μM Se. Chlorophyll fluorescence parameters were significantly lowered under Nd conditions but restored completely by Se addition reaching values of those in Na plants. Net CO2 assimilation rate (P N) decreased only slightly by limited N availability, but it enhanced significantly in both Nd and Na plants equally by 10 and 50 μM Se. Effect of Se on P N in the Na plants occurred mainly due to the stomata opening, while it was related to both stomatal and nonstomatal mechanisms in the Nd plants. The Se treatment resulted in enhancement of nitrate reductase (NR) activity in both Na and Nd plants with an optimal response at 10 μM Se. Negative correlations between nitrate concentration and NR activity indicated a partial nitrate depletion in the roots following by elevated NR activity in Nd plants. In contrast, nitrite concentrations were higher in the Se treated plants. Higher amino acids and protein concentrations in the Se-treated plants might be an indication of a general upregulation of N metabolism. However, in Na plants, the stimulation of N metabolism was not observed at 50 μM Se which could not be attributed to lesser availability of C skeletons because of maintaning higher CO2 fixation under these conditions. It implies the function of some regulatory mechanisms that are responsible for coordination of C and N metabolism in whole plant.

Additional key words: chlorophyll fluorescence; net assimilation rate; nitrate reductase; nutrients

Received: January 3, 2014; Accepted: March 4, 2014; Published: December 1, 2014  Show citation

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Hajiboland, R., & Sadeghzade, N. (2014). Effect of selenium on CO2 and NO3- assimilation under low and adequate nitrogen supply in wheat (Triticum aestivum L.). Photosynthetica, 52(4), 501-510. doi: 10.1007/s11099-014-0058-1
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    References

    1. Antal, T., Mattila, H., Hakala-Yatkin, M. et al.: Acclimation of photosynthesis to nitrogen deficiency in Phaseolus vulgaris. - Planta 232: 887-898, 2010. Go to original source...
    2. Bañuelos, G., Terry, N., Leduc, D.L. et al.: Field trial of transgenic Indian mustard plants shows enhanced phytoremediation of selenium-contaminated sediment. - Environ. Sci. Technol. 39: 1771-1777, 2005. Go to original source...
    3. Campbell, W.H.: Nitrate reductase structure, function and regulation: bridging the gap between biochemistry and physiology. - Annu. Rev. Plant Phys. 50: 277-303, 1999 Go to original source...
    4. Cataldo, D.A., Haroon, M., Schrader, L.E., Youngs, V.L.: Rapid colorimetric determination of nitrate in plant tissues by nitration of salicylic acid. - Commun. Soil Sci. Plan. 6: 71-80, 1975. Go to original source...
    5. Djanaguiraman, M., Devi, D.D., Shanker, A.K. et al.: Impact of selenium spray on monocarpic senescence of soybean (Glycine Max L.). - J. Food Agric. Environ. 2: 44-47, 2004
    6. Foyer, C.H., Parry, M., Noctor, G.: Markers and signals associated with nitrogen assimilation in higher plants. - J. Exp. Bot. 54: 585-593, 2003. Go to original source...
    7. Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    8. Germ, M., Stibilj, V., Kreft, I.: Metabolic importance of selenium for plants. - Eur. J. Plant Sci. Biotechnol. 1: 91-97, 2007.
    9. Habibi, G.: Effect of drought stress and selenium spraying on photosynthesis and antioxidant activity of spring barley. - Acta Agric. Slovenica 101: 31-39, 2013. Go to original source...
    10. Hajiboland, R., Amjad L.: Does antioxidant capacity of leaves play a role in growth response to selenium at different sulfur nutritional status? - Plant Soil Environ. 53: 207-215, 2007. Go to original source...
    11. Hajiboland, R., Keivanfar, N.: Selenium supplementation stimulates vegetative and reproductive growth in canola (Brassica napus L.) plants. - Acta Agric. Slovenica 99: 13-19, 2012. Go to original source...
    12. Hajiboland, R., Yang, X.E., Römheld, V.: Effects of bicarbonate and high pH on growth of Zn-efficient and Zn-inefficient genotypes of rice, wheat and rye. - Plant Soil 250: 349-357, 2003. Go to original source...
    13. Hasanuzzaman, M., Fujita, M.: Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings. - Acs. Sym. Ser. 143:1758-1776, 2011. Go to original source...
    14. Hawkesford, M., Horst, W., Kichey, T. et al.: Functions of macronutrients. - In: Marschner P. (ed.): Marschner's Mineral Nutrition of Higher Plants. Pp 135-189. Elsevier, Oxford 2012. Go to original source...
    15. Hawrylak-Nowak, B.: Beneficial effects of exogenous selenium in cucumber seedlings subjected to salt stress. - Acs. Sym. Ser. 132: 259-269, 2009. Go to original source...
    16. Heijari, J., Kivimäenpää, M., Hartikainen, H. et al.: Responses of strawberry (Fragaria×ananassa) to supplemental UV-B radiation and selenium under field conditions. - Plant Soil 282: 27-39, 2006. Go to original source...
    17. Heldt, H.W.: Plant Biochemistry. 3rd Ed. Pp 275-360. Elsevier Academic Press, Burlington 2005. Go to original source...
    18. Hirai, M.Y., Yano, M., Goodenowe, D.B. et al.: Integration of transcriptomics and metabolomics for understanding of global responses to nutritional stresses in Arabidopsis thaliana. - P. Natl. Acad. Sci. USA 101: 10205-10210, 2004. Go to original source...
    19. Huang, Z.A., Jiang, D.A., Yang, Y. et al.: Effects of nitrogen deficiency on gas exchange, chlorophyll fluorescence, and antioxidant enzymes in leaves of rice plants. - Photosynthetica 42: 357-364, 2004. Go to original source...
    20. Hwang, M., Ederer, G.M.: Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. - J. Clin. Microbiol. 1: 114-115, 1975. Go to original source...
    21. Jaworski, E.G.: Nitrate reductase assay in intact plant tissues. - Biochem. Bioph. Res. Co. 43: 1274-1279, 1971. Go to original source...
    22. Kaiser, W.M., Huber, S.C.: Post-translational regulation of nitrate reductase in higher plants. - Plant Physiol. 106: 817-821, 1994. Go to original source...
    23. Kopriva, S., Rennenberg, H.: Control of sulphate assimilation and glutathione synthesis: interaction with N and C metabolism. - J. Exp. Bot. 55: 1831-1842, 2004. Go to original source...
    24. Kraiser, T., Gras, D.E., Gutierrez, A.G. et al.: A holistic view of nitrogen acquisition in plants. - J. Exp. Bot. 62: 1455-1466, 2011. Go to original source...
    25. Lawlor, D.W.: Carbon and nitrogen assimilation in relation to yield: mechanisms are the key to understanding production systems. - J. Exp. Bot. 53: 773-787, 2002. Go to original source...
    26. Lee, J., Finley, J.W., Harnly, J.A.: Effect of selenium fertilizer on free amino acid composition of broccoli (Brassica oleracea cv. Majestic) determined by gas chromatography with flame ionization and mass selective detection. - J. Agr. Food Chem. 53: 9105-9111, 2005. Go to original source...
    27. Lichtenthaler, H.K., Wellburn, A.R.: Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. - Biol. Soc. Trans. 11: 591-592, 1983. Go to original source...
    28. Lima, J.D., Mosquim, P.R., Da Matta F.M.: Leaf gas exchange and chlorophyll fluorescence parameters in Phaseolus vulgaris as affected by nitrogen and phosphorus deficiency. - Photosynthetica 37: 113-121, 1999. Go to original source...
    29. Lu, C.M., Zhang, J.H.: Photosynthetic CO2 assimilation, chlorophyll fluorescence and photoinhibition as affected by nitrogen deficiency in maize plants. - Plant Sci. 151: 135-143, 2000. Go to original source...
    30. Luo, J., Li, H., Liu, T.X. et al.: Nitrogen metabolism of two contrasting poplar species during acclimation to limiting nitrogen availability. - J. Exp. Bot. 64: 4207-4224, 2013. Go to original source...
    31. Magné, C., Saladin, G., Clément, C.: Transient effect of the herbicide flazasulfuron on carbohydrate physiology in Vitis vinifera L.. - Chemosphere 62: 650-657, 2006. Go to original source...
    32. Masclaux-Daubresse, C., Daniel-Vedele, F., Dechorgnat, J. et al.: Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture. - Ann. Bot.-London 105: 1141-1157, 2010 Go to original source...
    33. Morcuende, R., Krapp, A., Hurry, V., Stitt, M.: Sucrose feeding leads to increased rates of nitrate assimilation, increased rates of α-oxoglutarate synthesis, and increased synthesis of a wide spectrum of amino acids in tobacco leaves. - Planta 206: 394-409, 1998. Go to original source...
    34. Müller, P., Li, X.P., Niyogi, K.K.: Non-photochemical quenching. A response to excess light energy. - Plant Physiol. 125: 1558-1566, 2001. Go to original source...
    35. Niyogi, K.K.: Photoprotection revisited: genetic and molecular approaches. - Annu. Rev. Plant Phys. 50: 333-359, 1999. Go to original source...
    36. Nunes-Nesi, A., Fernie, A.R., Stitt, M.: Metabolic and signaling aspects underpinning the regulation of plant carbon nitrogen interactions. - Mol. Plant 3: 973-996, 2010. Go to original source...
    37. Owusu-Sekyere, A., Kontturi, J., Hajiboland, R. et al.: Influence of selenium (Se) on carbohydrate metabolism, nodulation and growth in alfalfa (Medicago sativa L.). - Plant Soil 373: 541-552, 2013. Go to original source...
    38. Pennanen, A., Xue, T., Hartikainen, H.: Protective role of selenium in plant subjected to severe UV irradiation stress. - J. Appl. Bot.-Angew. Bot. 76: 66-76, 2002.
    39. Pilon-Smits, E.A.H., Quinn, C.F., Tapken, W. et al.: Physiological functions of beneficial elements. - Curr. Opin. Plant Biol. 12: 267-274, 2009. Go to original source...
    40. Pompelli, M.F., Martins, S.C.V., Antunes, W.C. et al.: Photosynthesis and photoprotection in coffee leaves is affected by nitrogen and light availabilities in winter conditions. - J. Plant Physiol. 167: 1052-1060, 2010. Go to original source...
    41. Reguera, M., Peleg, Z., Abdel-Tawab, Y.M. et al.: Stress-induced cytokinin synthesis increases drought tolerance through the coordinated regulation of carbon and nitrogen assimilation in rice. - Plant Physiol. 163: 1609-1622, 2013. Go to original source...
    42. Ruiz, J.M., Romero, L.: Relationship between potassium fertilisation and nitrate assimilation in leaves and fruits of cucumber (Cucumis sativus) plants. - Ann. Appl. Biol. 140: 241-245, 2002. Go to original source...
    43. Shen, J.B., Li, C.J., Mi, G.H. et al.: Maximizing root/rhizosphere efficiency to improve crop productivity and nutrient use efficiency in intensive agriculture of China. - J. Exp. Bot. 64: 1181-1192, 2013. Go to original source...
    44. Shrestha, S., Brueck, H., Asch, F.: Chlorophyll index, photochemical reflectance index and chlorophyll fluorescence measurements of rice leaves supplied with different N levels. - J. Photoch. Photobio. B 113: 7-13, 2012. Go to original source...
    45. Stitt, M., Müller, C., Matt, P. et al.: Steps towards an integrated view of nitrogen metabolism. - J. Exp. Bot. 53: 959-970, 2002. Go to original source...
    46. Tamaoki, M., Freeman, J.I., Marquès, L., Pilon-Smits, E.A.H.: New insights into the roles of ethylene and jasmonic acid in the acquisition of selenium resistance in plants. - Plant Signal. Behav. 3: 865-867, 2008. Go to original source...
    47. Terry, N., Zayed, A.M., de Souza, M.P., Tarun, A.S.: Selenium in higher plants. - Annu. Rev. Plant Phys. 51: 401-432, 2000. Go to original source...
    48. Xu, Z.C., Zhao, S., Jiao, J.H. et al.: Effects of different selenium concentrations on invertase and nitrate reductase activities of flue-cured tobacco. - Food Agric. Environ. 11: 964-967, 2013.
    49. Yao, X.Q., Chu, J.Z., Wang, G.Y.: Effects of selenium on wheat seedlings under drought stress. - Acs. Sym. Ser. 130: 283-290, 2009. Go to original source...
    50. Zhang, Y.Q., Liu, Z.J., Liu, R.Z. et al.: Gibberellins negatively regulate light-induced nitrate reductase activity in Arabidopsis seedlings. - J. Plant Physiol. 168: 2161-2168, 2011. Go to original source...

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