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Comparison of Open-Flow Microperfusion and Microdialysis Methodologies When Sampling Topically Applied Fentanyl and Benzoic Acid in Human Dermis Ex Vivo

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Abstract

Purpose

The purpose of this study is to compare two sampling methods—dermal Open-Flow Microperfusion (dOFM) and dermal Microdialysis (dMD) in an international joint experiment in a single-laboratory setting. We used human ex-vivo skin and sampled topically administered Fentanyl and Benzoic Acid. The second purpose was to provide guidance to researchers in choosing the most efficient method for a given penetrant and give suggestions concerning critical choices for successful dermal sampling.

Methods

The dOFM and dMD techniques are compared in equal set-ups using three probe-types (one dOFM probe and two dMD probe-types) in donor skin (n = 9) - 27 probes of each type sampling each penetrant in solutions applied in penetrationchambers glued to the skin surface over a time range of 20 h.

Results

Pharmacokinetic results demonstrated concordance between dOFM and dMD sampling technique under the given experimental conditions. The methods each had advantages and limitations in technical, practical and hands-on comparisons.

Conclusion

When planning a study of cutaneous penetration the advantages and limitations of each probe-type have to be considered in relation to the scientific question posed, the physico-chemical characteristics of the substance of interest, the choice of experimental setting e.g. ex vivo/in vivo and the analytical skills available.

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ACKNOWLEDGMENTS & DISCLOSURES

The authors thank the laboratory team in Denmark: Kim Brøsen and Flemming Nielsen for supervising the analysis and Birgitte Damby and Marie Mørk for technical assistance.

We also thank the laboratory team in Austria: Agnes Prasch and Anton Mautner for analysis and Katrin Tiffner, Simon Schwingenschuh, and Dina Tutkur for technical assistance during the experiment.

R.H. holds a research grant from The Danish Agency of Science Technology and Innovation. This study was supported in part by grants from Aage Bang’s Foundation, Else and Mogens Wedell-Wedellsborgs Foundation, Aase and Ejnar Danielsens Foundation, which are gratefully acknowledged.

The authors acknowledge the European COST-project ‘SkinBad (www.skinbad.eu)’ that facilitated the communication about the evaluation of in vivo methods for skin research.

Author information

Authors and Affiliations

  1. Department of Environmental Medicine, University of Southern Denmark, Odense, Denmark

    R. Holmgaard & J. B. Nielsen

  2. Department of Dermatology, Faculty of Health Sciences, University of Copenhagen, Roskilde Hospital, Roskilde, Denmark

    E. Benfeldt

  3. Department of Plastic and Reconstructive Surgery, Odense University Hospital, Odense, Denmark

    J. A. Sorensen

  4. Department of Endocrinology and Metabolism, Medical University Graz, Graz, Austria

    T. R. Pieber & F. Sinner

  5. Joanneum Research GmbH, HEALTH-Institute for Biomedicine and Health Sciences, Graz, Austria

    C. Gatschelhofer, C. Höfferer, M. Bodenlenz, T. R. Pieber & F. Sinner

  6. Department of Plastic Surgery, University of Copenhagen, Roskilde Hospital, Køgevej 7-9, 4000, Roskilde, Denmark

    R. Holmgaard

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  1. R. Holmgaard
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Correspondence to R. Holmgaard.

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Holmgaard, R., Benfeldt, E., Nielsen, J.B. et al. Comparison of Open-Flow Microperfusion and Microdialysis Methodologies When Sampling Topically Applied Fentanyl and Benzoic Acid in Human Dermis Ex Vivo . Pharm Res 29, 1808–1820 (2012). https://doi.org/10.1007/s11095-012-0705-9

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  • DOI: https://doi.org/10.1007/s11095-012-0705-9

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