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HPLC Assay of Rutin Content in the Leaves of the Black Poplar

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A method for assay of rutin content in the leaves of the black poplar (Populus nigra L.) using HPLC at a wavelength of 360 nm was developed; this used an Elsico column (No. 10302, 2 mm × 80 mm; Silasorb C18 7 μm), with a mobile phase consisting of acetonitrile-water (2:8) supplemented with 1% glacial acetic acid. The suitability of the method for analysis of standard rutin samples was confirmed. Rutin content in black poplar leaves ranged from 0.67% to 1.05%. Statistical analysis of the experimental results provided evidence that the error level in single determinations of rutin in black poplar leaves with a 95% confidence interval was ± 4.6%.

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Authors and Affiliations

  1. Samara State Medical University, 443099, Samara, Russia

    V. A. Kurkin & E. A. Kupriyanova

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  1. V. A. Kurkin
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  2. E. A. Kupriyanova
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Correspondence to V. A. Kurkin.

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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 54, No. 7, pp. 33 – 36, July, 2020.

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Kurkin, V.A., Kupriyanova, E.A. HPLC Assay of Rutin Content in the Leaves of the Black Poplar. Pharm Chem J 54, 717–720 (2020). https://doi.org/10.1007/s11094-020-02261-4

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