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Non-thermal Plasma Induced Expression of Heat Shock Factor A4A and Improved Wheat (Triticum aestivum L.) Growth and Resistance Against Salt Stress

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Abstract

There is a huge interest in making and applying innovating functional devices based on basic sciences (like physics) to improve plant growth and resistance against various stress conditions. This research was carried out in order to investigate effects of cold plasma on expressions of heat shock factor A4A (HSFA4A), plant growth and post reactions to salt stress. Wheat seedlings were treated with plasma (0.84 W/cm2 surface power densities) at different exposure times. In both three and 6 h after plasma, inductions in expressions of HSFA4A were recorded in roots, compared to control. Six hours after treatments, plasma-induced the shoot expressions of HSFA4A in the treated seedlings, contrasted to 3 h. Plasma treatment caused not the only enhancement in shoot fresh and dry mass and total leaf area, but also alleviated adverse impacts of salinity. Destroying impacts of salinity on chlorophyll contents were mitigated by plasma. Peroxidase activity was decreased by 27% for salinity treatment alone over control, while it was increased by 15% for plasma and salinity-treated samples, compared to salinity control. The highest activities of phenylalanine ammonia lyase (PAL) were found in plasma treatment alone. PAL activity was found to be higher in plasma-pretreated seedlings counteracted to salt stress, relative to the salinity control. The plasma treatment may act as an effective elicitor to modify gene expression, thereby improving plant growth and resistance. Plasma technology should be considered as a new functional technology in plant sciences.

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Acknowledgements

Authors would like to thank Dr. Mostafa Ebadi, and M.Sc. Hamed Nikmaram, M.Sc. Maryam Amini for their benevolent and professional collaborations in the research procedure. Corresponding author specially would like to acknowledge of Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, 1477893855, Iran

    Alireza Iranbakhsh & Narges Oraghi Ardebili

  2. Department of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Iran

    Zahra Oraghi Ardebili

  3. Department of Biology, Hamedan Branch, Islamic Azad University, Hamedan, Iran

    Mohammadreza Shafaati

  4. Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mahmood Ghoranneviss

Authors
  1. Alireza Iranbakhsh
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  2. Narges Oraghi Ardebili
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  3. Zahra Oraghi Ardebili
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  4. Mohammadreza Shafaati
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  5. Mahmood Ghoranneviss
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Correspondence to Alireza Iranbakhsh.

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Iranbakhsh, A., Ardebili, N.O., Ardebili, Z.O. et al. Non-thermal Plasma Induced Expression of Heat Shock Factor A4A and Improved Wheat (Triticum aestivum L.) Growth and Resistance Against Salt Stress. Plasma Chem Plasma Process 38, 29–44 (2018). https://doi.org/10.1007/s11090-017-9861-3

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  • DOI: https://doi.org/10.1007/s11090-017-9861-3

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