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Spectroscopic analysis of breath ethylene and oxidative stress relation with glycaemic status in type 2 diabetes

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Abstract

The paper presents the CO2 laser absorption spectroscopy (CO2LPAS) techniques as an effective tool for sensitive analysis of ethylene in human breath. Breath analysis is non-invasive method to monitor the disease states and evolution using biomarkers. Breath ethylene as a breath biomarker of oxidative stress was determined in the exhaled breath of subjects with type-2 diabetes and a healthy control group using a CO2LPAS system. Characterization of the CO2LPAS, and the selection of the ethylene absorption lines are described. All subjects involved in this study were subjected to breath analysis for determining the concentration of ethylene and blood tests to determine glucose levels and glycated hemoglobin A1c (HbA1c). Breath ethylene was found in high concentration in diabetics compared to healthy subjects and higher in subjects with type-2 diabetes with health complications. The data obtained by the CO2LPAS system suggest the involvement of oxidative stress in the complications of diabetes, revealing that the increased breath ethylene has a close relationship with high glucose levels, as observed by the HbA1c levels. The CO2LPAS system present a high sensitivity and high selectivity with fast measurement and can be used to monitor the molecular changes in diabetic subjects by measuring breath ethylene concentrations.

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Acknowledgement

We acknowledge the financial support to Project No. LAPLAS 4/PN 1647.

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Authors and Affiliations

  1. Department of Lasers, National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor St., P.O. Box MG-36, 077125, Bucharest, Romania

    Mioara Petrus, Ana-Maria Bratu & Cristina Popa

  2. University POLITEHNICA of Bucharest, 313 Splaiul Independentei St., Bucharest, Romania

    Cristina Popa

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  1. Mioara Petrus
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  2. Ana-Maria Bratu
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Correspondence to Mioara Petrus.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the author.

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This article is part of the Topical Collection on Laser technologies and laser applications.

Guest Edited by José Figueiredo, José Rodrigues, Nikolai A. Sobolev, Paulo André and Rui Guerra.

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Petrus, M., Bratu, AM. & Popa, C. Spectroscopic analysis of breath ethylene and oxidative stress relation with glycaemic status in type 2 diabetes. Opt Quant Electron 49, 2 (2017). https://doi.org/10.1007/s11082-016-0837-y

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