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Sulphated Fucooligosaccharide from Sargassum Horneri: Structural Analysis and Anti-Alzheimer Activity

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Abstract

In the present study, sulfated polysaccharides were obtained by digestion of Sargassum horneri and preparation with enzyme-assisted extraction using three food-grade enzymes, and their anti- Alzheimer’s activities were investigated. The results demonstrated that the crude sulfated polysaccharides extracted using AMGSP, CSP and VSP dose-dependently (25–100 µg·mL− 1) raised the spontaneous alternating manner (%) in the Y maze experiment of mice and reduced the escape latency time in Morris maze test. AMGSP, CSP and VSP also exhibited good anti-AChE and moderate anti-BuChE activities. CSP displayed the best inhibitory efficacy against AChE. with IC50 values of 9.77 µM. And, CSP also exhibited good inhibitory selectivity of AChE over BuChE. Next, CSP of the best active crude extract was separated by the preparation type high performance liquid phase to obtain the sulphated fucooligosaccharide section: SFcup (→3-α-L-fucp(2-SO3−)-1→4-α-L-fucp(2,3-SO3−)-1→section), SFcup showed a best inhibitory efficacy against AChE with IC50 values of 4.03 µM. The kinetic research showed that SFcup inhibited AChE through dual binding sites. Moreover, the molecular docking of SFcup at the AChE active site was in accordance with the acquired pharmacological results.

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Acknowledgements

We thank Sihong Wang from Yanbian University, China, for spectrum testing and spectrum analysis in this article. We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac), for editing the English text of a draft of the article.

Funding

The work was funded by Fujian Provincial Key Laboratory of Innovative Drug Target Research (NO. FJ-YW-2023KF04) and Zhejiang Ocean University Undergraduate Innovation Project (Academic Papers, 2022).

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Author notes

  1. Ling-Jian Zhang and Hao-Zheng Zhang contributed equally to this work.

Authors and Affiliations

  1. Food and Pharmacy College, Zhejiang Ocean University, Zhejiang, Zhoushan, 316022, China

    Ling-Jian Zhang, Hao-Zheng Zhang, Ya-Wen Liu, Min Tang, Yong-Jun Jiang & Li-Ping Guan

  2. Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Science, Xiamen University, Xiamen, 361102, China

    Fu-Nan Li

  3. College of Nursing, Zhejiang Pharmaceutical University, Zhejiang, Ningbo, 315153, China

    Qing-Hao Jin

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Contributions

L.P., Y.J. and QH were Conceptualization, Writing-original draft, Writing-review & editing; L.J., H.Z., F.N., and Y.W. prepared Investigation, Finishing the Experiments, and Data Analysis; T. and M. Assisted Execution Experiments. All authors reviewed the manuscript.

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Correspondence to Li-Ping Guan or Qing-Hao Jin.

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Zhang, LJ., Zhang, HZ., Liu, YW. et al. Sulphated Fucooligosaccharide from Sargassum Horneri: Structural Analysis and Anti-Alzheimer Activity. Neurochem Res 49, 1592–1602 (2024). https://doi.org/10.1007/s11064-024-04107-x

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