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H2S Attenuates Sleep Deprivation-Induced Cognitive Impairment by Reducing Excessive Autophagy via Hippocampal Sirt-1 in WISTAR RATS

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Abstract

Sleep deprivation (SD) is widespread in society causing serious damage to cognitive function. Hydrogen sulfide (H2S), the third gas signal molecule, plays important regulatory role in learning and memory functions. Inhibition of excessive autophagy and upregulation of silent information regulator 1 (Sirt-1) have been reported to prevent cognitive dysfunction. Therefore, this present work was to address whether H2S attenuates the cognitive impairment induced by SD in Wistar rats and whether the underlying mechanisms involve in inhibition of excessive autophagy and upregulation of Sirt-1. After treatment with SD for 72 h, the cognitive function of Wistar rats was evaluated by Y-maze, new object recognition, object location, and Morris water maze tests. The results shown that SD-caused cognitive impairment was reversed by treatment with NaHS (a donor of H2S). NaHS also prevented SD-induced hippocampal excessive autophagy, as evidenced by the decrease in autophagosomes, the down-regulation of Beclin1, and the up-regulation of p62 in the hippocampus of SD-exposed Wistar rats. Furthermore, Sirtinol, an inhibitor of Sirt-1, reversed the inhibitory roles of NaHS in SD-induced cognitive impairment and excessive hippocampal autophagy in Wistar rats. Taken together, our results suggested that H2S improves the cognitive function of SD-exposed rats by inhibiting excessive hippocampal autophagy in a hippocampal Sirt-1-dependent way.

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Data Availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (81771178), Natural Science Foundation of Hunan province (2019JJ80101), and the Major Research Topics of the Health Commission of Hunan province (20201911).

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Author notes

  1. Shan Gao, Yi-Yun Tang, and Li Jiang are contributed equally to this work.

Authors and Affiliations

  1. Hengyang Key Laboratory of Neurodegeneration and Cognitive Impairment, Institute of Neuroscience, Hengyang Medical College, University of South China, 28 W Changsheng Road, Hengyang, 421001, Hunan, P. R. China

    Shan Gao, Yi-Yun Tang, Li Jiang, Ping Zhang, Wei Zou, Yong-Jun Chen & Xiao-Qing Tang

  2. Department of Neurology, Affiliated Nanhua Hospital, University of South China, 336 E Dongfeng Road, Hengyang, 421001, Hunan, P. R. China

    Li Jiang, Fang Lan, Ping Zhang, Wei Zou & Yong-Jun Chen

  3. Institute of Neurology, the First Affiliated Hospital, University of South China, Hengyang, 421001, Hunan, P. R. China

    Fang Lan & Xiao-Qing Tang

  4. Department of Anesthesiology, the First Affiliated Hospital, University of South China, Hengyang, 421001, Hunan, P. R. China

    Xiang Li

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  1. Shan Gao
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Gao, S., Tang, YY., Jiang, L. et al. H2S Attenuates Sleep Deprivation-Induced Cognitive Impairment by Reducing Excessive Autophagy via Hippocampal Sirt-1 in WISTAR RATS. Neurochem Res 46, 1941–1952 (2021). https://doi.org/10.1007/s11064-021-03314-0

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