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Upregulation of PLZF is Associated with Neuronal Injury in Lipopolysaccharide-Induced Neuroinflammation

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Abstract

Promyelocytic leukemia zinc finger (PLZF) protein has been identified as a tumor suppressor in a variety of cancers, including leukemia, malignant mesothelioma, malignant melanoma, pancreatic cancer and prostate cancer. Studies have demonstrated that altered expression of PLZF affected its biological functions associated with tumorigenesis, such as proliferation, cell cycle, and apoptosis. However, information regarding its regulation and possible function in the central nervous system diseases is still limited. In this study, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventricle injection in adult rats and detected increased expression of PLZF in the brain cortex. Immunofluorescence assay indicated that PLZF was significantly increased in neurons 3 day after LPS injection, but not in astrocytes and microglia. Moreover, there was a concomitant upregulation of active caspase-3, cyclin D1, and CDK4 in vivo and vitro studies. In addition, the expression of these proteins in cortical primary neurons was inhibited after knocking down PLZF by siRNA. Collectively, all these results suggested that the upregulation of PLZF might be involved in neuronal apoptotic-like injury in neuroinflammation after LPS injection.

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Acknowledgments

This work was supported by the National Natural Scientific Foundation of China (No. 81270428).

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Authors and Affiliations

  1. Department of Geriatrics, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, Jiangsu, China

    Mingqing He & Xiang Lu

  2. Department of Geriatrics, The Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

    Mingqing He

  3. Department of Pathogen Biology Medical College, Nantong University, Nantong, 226001, Jiangsu, China

    Yonghua Liu

  4. Department of Neurology, The Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

    Jiabing Shen

  5. The second people’s hospital of Nantong, Nantong, 226002, Jiangsu, China

    Chengwei Duan

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  1. Mingqing He
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Correspondence to Xiang Lu.

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He, M., Liu, Y., Shen, J. et al. Upregulation of PLZF is Associated with Neuronal Injury in Lipopolysaccharide-Induced Neuroinflammation. Neurochem Res 41, 3063–3073 (2016). https://doi.org/10.1007/s11064-016-2027-5

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