Abstract
The adipocytokine apelin is a peptide, Apelin and its receptor are abundantly expressed in the nervous and cardiovascular systems. Previous studies had found apelin-13 reduces brain injuries and postischemic cerebral edema through blocking programmed cell death, Apelin-13 is also able to inhibit glucose deprivation induced cardiomyocyte autophagy in a concentration dependent fashion. To observe the effect of Apelin-13 on the brain injury induced by traumatic brain injury (TBI), and explore the effect of Apelin-13 on autophagy in TBI, We performed The neurological test, and the numbers of TBI-induced neural cell death were also counted by propidium iodide labeling. At last, the autophagy associated proteins LC3, Beclin-1, Bcl-2, p62 were also assessed with western-blotting. Compared with saline vehicle groups, the neural cell death, lesion volume, and neural dysfunction were attenuated by apelin-13 after TBI. In additionally, Apelin-13 also reversed TBI induced downregulation of LC3, Beclin-1, Bcl-2, p62 expression, compared with saline vehicle groups, at 24 and 48 h post TBI. Apelin-13 attenuates TBI induced brain damage by suppressing autophagy. All these results revealed that Apelin-13 suppressed autophagy. The autophagy may be involved in the mechanism of Apelin-13 rescue the subsequent damaged neuron in TBI.
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Abbreviations
- TBI:
-
Traumatic brain injury
- CCI:
-
Contolled cortical impact
- LC3:
-
Microtubule-associated protein 1 light chain 3
- PI:
-
Propidium iodide
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The work was supported by the National Science Foundation of China (No 81302612).
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Hai-Jun Bao and Lin Zhang have equally contributed to this work.
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Bao, HJ., Zhang, L., Han, WC. et al. Apelin-13 Attenuates Traumatic Brain Injury-Induced Damage by Suppressing Autophagy. Neurochem Res 40, 89–97 (2015). https://doi.org/10.1007/s11064-014-1469-x
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DOI: https://doi.org/10.1007/s11064-014-1469-x