Abstract
The Ca2+-dependent release of aspartate from hippocampal preparations was first reported 35 years ago, but the functional significance of this process remains uncertain. Aspartate satisfies all the criteria normally required for identification of a CNS transmitter. It is synthesized in nerve terminals, is accumulated and stored in synaptic vesicles, is released by exocytosis upon nerve terminal depolarization, and activates postsynaptic NMDA receptors. Aspartate may be employed as a neuropeptide-like co-transmitter by pathways that release either glutamate or GABA as their principal transmitter. Aspartate mechanisms include vesicular transport by sialin, vesicular content sensitive to glucose concentration, release mainly outside the presynaptic active zones, and selective activation of extrasynaptic NR1-NR2B NMDA receptors. Possible neurobiological functions of aspartate in immature neurons include activation of cAMP-dependent gene transcription and in mature neurons inhibition of CREB function, reduced BDNF expression, and induction of excitotoxic neuronal death. Recent findings suggest new experimental approaches toward resolving the functional significance of aspartate release.
Similar content being viewed by others
References
Bliss TVP, Lømo T (1973) Long-lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path. J Physiol 232:331–356
Bliss TVP, Gardner-Medwin AR (1973) Long-lasting potentiation of the dentate area in the unanaesthetized rabbit following stimulation of the perforant path. J Physiol 232:357–374
Cotman CW, Nadler JV (1978) Reactive synaptogenesis in the hippocampus. In: Cotman CW (ed) Neuronal plasticity. Raven, New York, pp 227–271
Curtis DR, Phillis JW, Watkins JC (1960) The chemical excitation of spinal neurones by certain acidic amino acids. J Physiol 150:656–682
Curtis DR, Watkins JC (1960) The excitation and depression of spinal neurones by structurally related amino acids. J Neurochem 6:117–141
Nadler JV, Vaca KW, White WF et al (1976) Aspartate and glutamate as possible transmitters of excitatory hippocampal afferents. Nature 260:538–540
Nadler JV, White WF, Vaca KW et al (1977) Characterization of putative amino acid transmitter release from slices of rat dentate gyrus. J Neurochem 29:279–290
Gundersen V, Ottersen OP, Storm-Mathisen J (1991) Aspartate- and glutamate-like immunoreactivities in rat hippocampal slices: depolarization-induced redistribution and effects of precursors. Eur J Neurosci 3:1281–1299
Gundersen V, Chaudhry FA, Bjaalie JG et al (1998) Synaptic vesicular localization and exocytosis of L-aspartate in excitatory nerve terminals: a quantitative immunogold analysis in rat hippocampus. J Neurosci 18:6059–6070
Gundersen V, Holten AT, Storm-Mathisen J (2004) GABAergic synapses in hippocampus exocytose aspartate on to NMDA receptors: quantitative immunogold evidence for co-transmission. Mol Cell Neurosci 26:156–165
Larsson M, Persson S, Ottersen OP et al (2001) Quantitative analysis of immunogold labeling indicates low levels and non-vesicular localization of L-aspartate in rat primary afferent terminals. J Comp Neurol 430:147–159
Persson S, Broman J (2004) Glutamate, but not aspartate, is enriched in trigeminothalamic tract terminals and associated with their synaptic vesicles in the rat nucleus submedius. Exp Brain Res 157:152–161
Gundersen V, Fonnum F, Ottersen OP et al (2001) Redistribution of neuroactive amino acids in hippocampus and striatum during hypoglycemia: a quantitative immunogold study. J Cereb Blood Flow Metab 21:41–51
Burke SP, Nadler JV (1988) Regulation of glutamate and aspartate release from slices of the hippocampal CA1 area: effects of adenosine and baclofen. J Neurochem 51:1541–1551
Zhou M, Peterson CL, Lu Y-B et al (1995) Release of glutamate and aspartate from CA1 synaptosomes: selective modulation of aspartate release by ionotropic glutamate receptor ligands. J Neurochem 64:1556–1566
Bradford SE, Nadler JV (2004) Aspartate release from rat hippocampal synaptosomes. Neuroscience 128:751–765
Nicholls D, Attwell D (1990) The release and uptake of excitatory amino acids. Trends Pharmacol Sci 11:462–468
Peterson CL, Thompson MA, Martin D et al (1995) Modulation of glutamate and aspartate release from slices of hippocampal area CA1 by inhibitors of arachidonic acid metabolism. J Neurochem 64:1152–1160
Herrero MT, Oset-Gasque MJ, Cañadas C et al (1998) Effect of various depolarizing agents on endogenous amino acid transmitter release in rat cortical neurons in culture. Neurochem Int 32:257–264
Fleck MW, Barrionuevo G, Palmer AM (2001) Synaptosomal and vesicular accumulation of L-glutamate, L-aspartate and D-aspartate. Neurochem Int 39:217–225
Cavallero A, Marte A, Fedele E (2009) L-Aspartate as an amino acid neurotransmitter: mechanisms of the depolarization-induced release from cerebrocortical synaptosomes. J Neurochem 110:924–934
Iwamoto T, Watano T, Shigekawa M (1996) A novel isothiourea derivative selectively inhibits the reverse mode of Na+/Ca2+ exchange in cells expressing NCX1. J Biol Chem 271:22391–22397
Holten AT, Morland C, Nordengen K et al (2008) Vesicular release of L- and D-aspartate from hippocampal nerve terminals: immunogold evidence. Open Neurosci J 2:51–58
McMahon HT, Foran P, Dolly JO et al (1992) Tetanus toxin and botulinum toxins type A and B inhibit glutamate, γ-aminobutyric acid, aspartate, and met-enkephalin release from synaptosomes. J Biol Chem 267:21338–21343
Fleck MW, Barrionuevo G, Palmer AM (2001) Release of D-threo-β-hydroxyaspartate as a false transmitter from excitatory amino acid-releasing nerve terminals. Neurochem Int 39:75–81
Rossetto O, Morbiato L, Caccin P et al (2006) Presynaptic enzymatic neurotoxins. J Neurochem 97:1534–1545
Schiavo G, Matteoli M, Montecucco C (2000) Neurotoxins affecting neuroexocytosis. Physiol Rev 80:717–766
Simpson LL (2004) Identification of the major steps in botulinum neurotoxin action. Annu Rev Pharmacol Toxicol 44:167–193
Wang L, Nadler JV (2007) Reduced aspartate release from rat hippocampal synaptosomes loaded with Clostridial toxin light chain by electroporation: evidence for an exocytotic mechanism. Neurosci Lett 412:239–242
Sobolevsky AI, Khodorov BI (1999) Blockade of NMDA channels in acutely isolated rat hippocampal neurons by the Na+/Ca2+ exchange inhibitor KB-R7943. Neuropharmacology 38:1235–1242
Arawaka N, Sakaue M, Yokoyama I et al (2000) KB-R7943 inhibits store-operated Ca2+ entry in cultured neurons and astrocytes. Biochem Biophys Res Comm 279:354–357
Pintado AJ, Herrero CJ, Garcia AG et al (2000) The novel Na+/Ca2+ exchange inhibitor KB-R7943 also blocks native and expressed neuronal nicotinic receptors. Brit J Pharmacol 130:1893–1902
Kraft R (2007) The Na+/Ca2+ exchange inhibitor KB-R7943 potently blocks TRPC channels. Biochem Biophys Res Comm 361:230–236
Liang GH, Kim JA, Seol GH et al (2008) The Na+/Ca2+ exchange inhibitor KB-R7943 activates large-conductance Ca2+-activated K+ channels in endothelial and vascular smooth muscle cells. Eur J Pharmacol 582:35–41
Chung YH, Ahn HS, Kim D et al (2006) Immunohistochemical study on the distribution of TRPC channels in the rat hippocampus. Brain Res 1085:132–137
Zhou J, Du W, Zhou K et al (2008) Critical role of TRPC6 channels in the formation of excitatory synapses. Nat Neurosci 11:741–743
Szerb JC (1988) Changes in the relative amounts of aspartate and glutamate released and retained in hippocampal slices during stimulation. J Neurochem 50:219–224
McNay EC, Gold PE (1999) Extracellular glucose concentrations in the rat hippocampus measured by zero-net-flux: effects of microdialysis flow rate, strain and age. J Neurochem 72:785–790
Burke SP, Nadler JV (1989) Effects of glucose deficiency on glutamate/aspartate release and excitatory synaptic responses in the hippocampal CA1 area in vitro. Brain Res 500:333–342
Fleck MW, Henze DA, Barrionuevo G et al (1993) Aspartate and glutamate mediate excitatory synaptic transmission in area CA1 of the hippocampus. J Neurosci 13:3944–3955
Reimer RJ, Edwards RH (2004) Organic anion transport is the primary function of the SLC17/type 1 phosphate transporter family. Pflügers Arch 447:629–635
Miyaji T, Echigo N, Hiasa M et al (2008) Identification of a vesicular aspartate transporter. Proc Natl Acad Sci USA 105:11720–11724
Prolo LM, Vogel H, Reimer RJ (2009) The lysosomal sialic acid transporter sialin is required for normal CNS myelination. J Neurosci 29:15355–15365
Li D, Ropert N, Koulakoff A et al (2008) Lysosomes are the major vesicular compartment undergoing Ca2+-regulated exocytosis from cortical astrocytes. J Neurosci 28:7648–7658
Aula N, Kopra O, Jalanko A et al (2004) Sialin expression in the CNS implicates extralysosomal function in neurons. Neurobiol Dis 15:251–261
Yatsushiro S, Yamada H, Kozaki S et al (1997) L-Aspartate but not the D form is secreted through microvesicle-mediated exocytosis and is sequestered through Na+-dependent transporter in rat pinealocytes. J Neurochem 69:340–347
Yarovaya N, Schot R, Fodero L et al (2005) Sialin, an anion transporter defective in sialic acid storage diseases, shows highly variable expression in adult mouse brain, and is developmentally regulated. Neurobiol Dis 19:351–365
Aula N, Salomäki P, Timonen R et al (2000) The spectrum of SLC17A5-gene mutations resulting in free sialic acid-storage diseases indicates some genotype-phenotype correlation. Am J Hum Genet 67:832–840
Morin P, Sagné C, Gasnier B (2004) Functional characterization of wild-type and mutant human sialin. EMBO J 23:4560–4570
Wreden CC, Wlizla M, Reimer RJ (2005) Varied mechanisms underlie the free sialic acid storage disorders. J Biol Chem 280:1408–1416
Patneau DK, Mayer ML (1990) Structure-activity relationships for amino acid transmitter candidates acting at N-methyl-D-aspartate and quisqualate receptors. J Neurosci 10:2385–2399
Curras MC, Dingledine R (1992) Selectivity of amino acid transmitters acting at N-methyl-D-aspartate and amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors. Mol Pharmacol 41:520–526
Frauli M, Neuville P, Vol C et al (2006) Among the twenty classical L-amino acids, only glutamate directly activates metabotropic glutamate receptors. Neuropharmacology 50:245–253
Thomas CG, Miller AJ, Westbrook GL (2006) Synaptic and extrasynaptic NMDA receptor NR2 subunits in cultured hippocampal neurons. J Neurophysiol 95:1727–1734
Erreger K, Geballe MT, Kristensen A et al (2007) Subunit-specific agonist activity at NR2A-, NR2B-, NR2C-, and NR2D-containing N-methyl-D-aspartate glutamate receptors. Mol Pharmacol 72:907–920
Fonnum F, Paulsen RH, Fosse VM et al (1986) Synthesis and release of amino acid transmitters. In: Schwarcz R, Ben-Ari Y (eds) Excitatory amino acids and epilepsy. Advances in Experimental medicine and biology, 203. Plenum, New York, pp 285–293
Szerb JC, O’Regan PA (1987) Reversible shifts in the Ca2+-dependent release of aspartate and glutamate from hippocampal slices with changing glucose concentrations. Synapse 1:265–272
Zhang X, Nadler JV (2009) Postsynaptic response to stimulation of the Schaffer collaterals with properties similar to those of synaptosomal aspartate release. Brain Res 1295:13–20
Almeida LEF, Murray PD, Zielke HR et al (2009) Autocrine activation of neuronal NMDA receptors by aspartate mediates dopamine- and cAMP-induced CREB-dependent gene transcription. J Neurosci 29:12702–12710
Hardingham GE, Fukunaga Y, Bading H (2002) Extrasynaptic NMDARs oppose synaptic NMDARs by triggering CREB shut-off and cell death pathways. Nat Neurosci 5:405–414
Vanhoutte P, Bading H (2003) Opposing roles of synaptic and extrasynaptic NMDA receptors in neuronal calcium signalling and BDNF gene regulation. Curr Opinion Neurobiol 13:366–371
Wahl A-S, Buchthal B, Rode F et al (2009) Hypoxic/ischemic conditions induce expression of the putative pro-death gene Clca1 via activation of extrasynaptic N-methyl-D-aspartate receptors. Neuroscience 158:344–352
Baird DH, Trenkner E, Mason CA (1996) Arrest of afferent axon extension by target neurons in vitro is regulated by the NMDA receptor. J Neurosci 16:2642–2648
Rocha M, Sur M (1995) Rapid acquisition of dendritic spines by visual thalamic neurons after blockade of N-methyl-D-aspartate receptors. Proc Natl Acad Sci USA 92:8026–8030
Nacher J, Rosell DR, Alonso-Llosa G et al (2001) NMDA receptor antagonist treatment induces a long-lasting increase in the number of proliferating cells, PSA-NCAM-immunoreactive granule neurons and radial glia in the adult rat dentate gyrus. Eur J Neurosci 13:512–520
Nadler JV, Evenson DA, Cuthbertson GJ (1981) Comparative toxicity of kainic acid and other acidic amino acids toward rat hippocampal neurons. Neuroscience 6:2505–2517
Breder J, Sabelhaus CF, Opitz T, Reymann KG, Schröder UH (2000) Inhibition of different pathways influencing Na+ homeostasis protects organotypic hippocampal slice cultures from hypoxic/hypoglycemic injury. Neuropharmacology 39:1779–1787
Schröder UH, Breder J, Sabelhaus CF, Reymann KG (1999) The novel Na+/Ca2+ exchange inhibitor KB-R7943 protects CA1 neurons in rat hippocampal slices against hypoxic/hypoglycemic injury. Neuropharmacology 38:319–321
Acknowledgments
I thank the many collaborators who contributed their efforts toward these studies. Special thanks go to Dianna Johnson, without whose collaboration the study of excitatory hippocampal transmitters might not have got off the ground, and Carl Cotman, who supported and encouraged this line of research. I also wish to acknowledge the generous support of my laboratory’s studies of aspartate transmission by grants NS 16064 and NS 47096 from the National Institutes of Health.
Author information
Authors and Affiliations
Corresponding author
Additional information
Special Issue: In Honor of Dr. Dianna Johnson.
Rights and permissions
About this article
Cite this article
Nadler, J.V. Aspartate Release and Signalling in the Hippocampus. Neurochem Res 36, 668–676 (2011). https://doi.org/10.1007/s11064-010-0291-3
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11064-010-0291-3