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Human Serotonin Transporter Expression During Megakaryocytic Differentiation of MEG-01 Cells

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Abstract

The serotonin (5-HT) transporter (SERT) has been found altered in platelets of patients with genetically complex disorders, including mood-anxiety, pain and eating disorders. In this study, we used cell cultures of platelet precursors as models of investigation on mechanisms of SERT regulation: SERT expression was appraised during megakaryocytic differentiation of human megakaryoblastic MEG-01 cells. Cells were cultured for 8 days with 10−7M 4-β-12-tetradecanoylphorbol-13-acetate (β-TPA) in the presence of 10% fetal bovine serum (FBS) and SERT was assessed by real time PCR, immunofluorescence microscopy, Western blot and [3H]5-HT re-uptake. Results revealed that SERT is present in control-untreated MEG-01 cells. β-TPA-differentiating MEG-01 cells showed a redistribution of SERT fluorescence, diffuse to cell bodies and blebs along with a 3-fold SERT mRNA increase and a moderate raise in SERT protein (1.5/1.4-fold) by immunoblot and re-uptake assays. In summary, we have shown herein that control megakaryoblasts express the SERT protein. SERT is modulated by differentiation events, implying that SERT density in platelets is under the control of megakaryocytopoiesis stages. Differentiation of MEG-01 cells can provide considerable insight into interactions between SERT genetics, transmitter-hormonal/homeostatic mechanisms and signaling pathways.

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Acknowledgments

The present work is supported by a grant of “Ministero dell’Istruzione dell’Università e della Ricerca” (M.I.U.R.) to Prof. G. Giannaccini.

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Authors and Affiliations

  1. Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Via Bonanno 6, 56126, Pisa, Italy

    Gino Giannaccini, Laura Betti, Lionella Palego, Lara Schmid, Laura Fabbrini, Claudia Gargini, Ylenia Da Valle, Mario Lanza & Antonio Lucacchini

  2. Department of Endocrinology and Metabolism, Sections of Endocrinology and Metabolism, Environment and Endocrine and Nervous Systems High Technology, Center for the Study of the Effects of Harmful Agents, University of Pisa, via Paradisa 2, 56124, Pisa, Italy

    Caterina Pelosini, Alessandro Marsili, Margherita Maffei, Ferruccio Santini, Paolo Vitti & Aldo Pinchera

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  1. Gino Giannaccini
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Correspondence to Gino Giannaccini.

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Giannaccini, G., Betti, L., Palego, L. et al. Human Serotonin Transporter Expression During Megakaryocytic Differentiation of MEG-01 Cells. Neurochem Res 35, 628–635 (2010). https://doi.org/10.1007/s11064-009-0112-8

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  • DOI: https://doi.org/10.1007/s11064-009-0112-8

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