Abstract
In the present study, we investigated the influences of a high fat diet (HD) fed for 12 weeks, on lipid peroxidation and antioxidant enzyme using 4-hydroxy-2E-nonenal (HNE)-modified proteins (HNE-mp) and Cu,Zn-superoxide dismutase (SOD1) in the hippocampal CA1 region (CA1) in C57BL/6N and C3H/HeN mice. Body weights and body weight gains were significantly higher in HD fed C57BL/6N mice than in low fat diet (LD) fed C57BL/6N and LD or HD fed C3H/HeN mice. In the HD fed C57BL/6N and C3H/HeN mice, HNE-mp immunoreactivity and protein levels were much higher than in the LD fed C57BL/6N or C3H/HeN mice. In particular, HNE-mp immunoreactivity and protein levels in HD fed C57BL/6N mice was higher than that in the HD fed C3H/HeN mice. SOD1 immunoreaction was detected in the non-pyramidal cells of C57BL/6N mice, while in the C3H/HeN mice SOD1 immunoreaction was observed in CA1 pyramidal cells. The SOD1 immunoreactivity in the LD fed C57BL/6N and C3H/HeN mice was slightly, but not significantly decreased compared to that in the HD fed C57BL/6N and C3H/HeN mice, respectively. In addition, ionized calcium-binding adapter molecule 1 (Iba-1) immunoreactive microglia in the HD fed C57BL/6N showed hypertrophy of cytoplasm, which is the characteristics of activated microglia. These results suggest that HD fed C57BL/6N mice are more susceptible to lipid peroxidation in the CA1 than in LD fed C57BL/6N and LD or HD fed C3H/HeN mice without any differences of SOD1 expression.
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Acknowledgements
This study was supported by a grant of the Oriental Medicine R&D Project, Ministry of Health and Welfare, Republic of Korea (HMP-00-CO-06-0006) and was also supported by the Grants for Medical Research Center for Chronic Metabolic Diseases, Yonsei University College of Medicine, from Korean Ministry of Health and Welfare to Seong JK.
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In Koo Hwang and Il Yong Kim have contributed equally to this article.
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Hwang, I.K., Kim, I.Y., Kim, Y.N. et al. Comparative Study on High Fat Diet-induced 4-Hydroxy-2E-nonenal Adducts in the Hippocampal CA1 Region of C57BL/6N and C3H/HeN Mice. Neurochem Res 34, 964–972 (2009). https://doi.org/10.1007/s11064-008-9846-y
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DOI: https://doi.org/10.1007/s11064-008-9846-y