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Protective Effect Against 17β-Estradiol on Neuronal Apoptosis in Hippocampus Tissue Following Transient Ischemia/Recirculation in Mongolian Gerbils via Down-Regulation of Tissue Transglutaminase Activity

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Abstract

We analyzed the protective effect of 17β-estradiol (17β-ED) injection against delayed neuronal death in the hippocampus tissue of the brain in Mongolian gerbils after transient ischemia/recirculation treatment, especially in relation with bcl-2 gene expression and enzymatic activity changes of caspase-3 and tissue transglutaminase (tTGase). Daily intraperitoneal injection of 17β-ED to the animal after the ischemia stimulated the expression of an apoptosis suppressor gene, bcl-2, in the hippocampal tissue for a week. The gradually increasing apoptotic enzyme activity of caspase-3 and increased number of TUNEL positive fragmented neuronal nuclei caused by ischemic attack in the gerbil brain were clearly suppressed by 17β-ED administration. The reduced activity and enzyme protein of tTGase, a neurodegenerative marker of apoptosis in the hippocampus after ischemia, were also restored to nearly normal levels by 17β-ED injection. These results suggest that daily 17β-ED administration to the gerbil after transient ischemic insult with progressing neuronal deteriorative changes in hippocampus tissue can effectively prevent apoptotic changes through a molecular cascade involving gene expression regulation.

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, Fujita Health University, Toyoake, Aichi , 470-1192, Japan

    K. Fujita, H. Imada, E. Miyachi & Y. Nagata

  2. Department of Clinical Laboratory Science, Fujita Health University College, Toyoake, Aichi , 470-1192, Japan

    T. Kato

  3. Department of Adult Nursing, Fujita Health University, Toyoake, Aichi , 470–1192, Japan

    K. Shibayama

  4. Department of Nutrition and Food Sciences, Aichi Gakusen University, Okazaki, Aichi , 444–8520, Japan

    M. Yamauchi

  5. Department of Nutrition and Food Sciences, Nagoya Bunri College, Nagoya, Aichi , 451–0077, Japan

    N. Yoshimoto

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  1. K. Fujita
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Correspondence to K. Fujita.

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Fujita, K., Kato, T., Shibayama, K. et al. Protective Effect Against 17β-Estradiol on Neuronal Apoptosis in Hippocampus Tissue Following Transient Ischemia/Recirculation in Mongolian Gerbils via Down-Regulation of Tissue Transglutaminase Activity. Neurochem Res 31, 1059–1068 (2006). https://doi.org/10.1007/s11064-006-9114-y

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