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Repeated Electroconvulsive Shock Induces Changes in High-affinity [3H]-ouabain Binding to Rat Striatal Membranes

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Repeated electroconvulsive shock is an effective treatment for affective disorders. Striatum, hippocampus and brainstem are involved in affective disorders. Sodium–potassium/ATPase is of paramount importance for the proper functioning of the brain and its involvement in the affective disorders has been claimed for a long time. Sodium–potassium/ATPase has an extracellular regulatory binding site to which cardiotonic glycosides, such as ouabain, bind to, thus regulating the activity of the enzyme. Endogenous “ouabain-like” substances exist in the brain and their actions on the sodium–potassium/ATPase resemble ouabain biological properties. The aim of this work was to determine if electroconvulsive shock (ECS) would induce changes in the high-affinity binding of ouabain to the sodium–potassium/ATPase from rat brain regions. Adult, male Wistar rats received one (ECS×1 group) or seven electroshocks (ECS×7 group) delivered daily through ear-clips electrodes. Control rats received the same manipulations; however, no current was delivered through the electrodes (SHAM×1 and SHAM×7 groups). All groups were sacrificed 24 h after the last ECS session. The B max and K D of high-affinity [3H]-ouabain binding were determined in crude membrane preparations from the striatum, hippocampus and brainstem. The results obtained showed a statistically significant increase in the affinity of [3H]-ouabain (lower K D) to striatal membranes in those rats receiving seven ECS. In the striatum there was no change in the K D after one ECS; as well as there was no change in the B max after a single or seven ECS. High-affinity [3H]-ouabain binding to hippocampus and brainstem did not reveal any significant differences either in K D or B max after one or seven ECS. The increased affinity of ouabain to the striatal sodium–potassium/ATPase induced by repeated ECS suggests an increased interaction in vivo of the endogenous “ouabain-like” substances with the enzyme and the involvement of the extracellular regulatory allosteric ouabain binding site in the striatal sodium–potassium/ATPase in the effects of electroconvulsive shock.

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Acknowledgements

We thank Marcela I.F. Venditti for her help in the preparation of this manuscript. Magda Bignotto was a recipient of a fellowship from Coordenação de Pessoal de Nível Superior (CAPES). This work was funded by Associação Fundo de Incentivo à Psicofarmacologia (AFIP).

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  1. Departamento de Psicobiologia, Universidade Federal de São Paulo, Rua Botucatu, 862-1° andar, 04023-900 , São Paulo, SP, Brazil

    Magda Bignotto & Marco Antonio Campana Benedito

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  1. Magda Bignotto
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  2. Marco Antonio Campana Benedito
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Correspondence to Marco Antonio Campana Benedito.

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Bignotto, M., Benedito, M.A.C. Repeated Electroconvulsive Shock Induces Changes in High-affinity [3H]-ouabain Binding to Rat Striatal Membranes. Neurochem Res 31, 515–521 (2006). https://doi.org/10.1007/s11064-006-9046-6

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