In in vitro experiments, we showed that hydrogen sulfide used in concentrations from 10-6 to 10–3 M caused dose-dependent relaxation of circular preparations of the aorta and mesenterial and renal arteries. With increase in the H2S concentration to 10–2 M, we observed intensification of the relaxing effect only with respect to preparations of mesenterial and renal arteries. Vasorelaxation of walls of the aorta and mesenterial and renal arteries induced by applications of cysteine (10–6 to 10–2 М) was completely inhibited in the presence of propargylglycine. Homocysteine used in relatively high concentrations (10–4 to 10–2 М) caused a decrease in the endothelium-dependent vasorelaxation induced by acetylcholine. The renal and mesenterial arteries demonstrated the maximum sensitivity to homocysteine, while the minimum sensitivity was demonstrated by the aorta. Pre-incubation of the vessels together with L-NAME and indomethacin decreased the vasorelaxing action of hydrogen sulfide, while pre-incubation of the studied vessels with sodium nitroprusside intensified such action.
Similar content being viewed by others
References
O. O. Pentyuk, M. B. Lutsyuk, I. I. Andrushko, and K. P. Postovitenko, “Metabolism of homocysteine and its role in pathology,” Ukr. Biokhim. Zh., 75, No. 1, 5-17 (2003).
I. I. Andrushko, “Content of cysteine in reasonably healthy persons and in patients with ischemic cardiac disease,” Ukr. Kardiol. Zh., No. 3, 43-47 (2007).
M. H. Stipanuk, J. E. Dominy Jr., J. I. Lee, and R. M. Coloso, “Mammalian cysteine metabolism: new insights into regulation of cysteine metabolism,” J. Nutr., 136, No. 6, 1652-1659 (2006).
W. Zhao, J. Zhang, Y. Lu, and R. Wang, “The vasorelaxant effect of H2S as a novel endogenous gaseous K(ATP) channel opener,” EMBO J., 20, No. 21, 6008-6016 (2001).
E. Łowicka and J.Bełtowski, “Hydrogen sulfide (H2S) – the third gas of interest for pharmacologists,” Pharmacol. Rep., 59, No. 1, 4-24 (2007).
R. Wang, “Two’s company, three’s a crowd: can H2S be the third endogenous gaseous transmitter?” FASEB J., 16, No. 13, 1792-1780 (2002).
A. V. Gurkovskaya, V. A. Bouryi, N. I. Gokina, and M. F. Shuba, “Electrophysiological analysis of excitatory action of serotonin on coronary arteries,” Fiziol. Zh.., 34, No. 5, 66-72 (1998).
M. N. Tkachenko, V, F. Sagach, O. V. Bazilyuk, et al., “The age peculiarities of changes in contractile vascular reactions and the content of free oxygen radicals and metabolites of nitric oxide in BALB/c mice under conditions of their stay in the zone of alienation,” Fiziol. Zh., 51, No. 3, 32-41 (2005).
G. E. Roitberg and A. V. Strutinskii, Internal Diseases. Cardiovascular System [in Russian], Meditsina, Moscow (2003).
Author information
Authors and Affiliations
Corresponding author
Additional information
Neirofiziologiya/Neurophysiology, Vol. 42, No. 2, pp.126-131, March-April, 2010.
Rights and permissions
About this article
Cite this article
Mel’nik, A.V., Voloshchouk, N.I., Pentyuk, N.O. et al. Role of Hydrogen Sulfide and Sulfur-Containing Amino Acids in Regulation of Tone of Smooth Muscles of the Vascular Wall in Rats. Neurophysiology 42, 104–109 (2010). https://doi.org/10.1007/s11062-010-9137-5
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11062-010-9137-5