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Exosome-loaded dendritic cells elicit tumor-specific CD8+ cytotoxic T cells in patients with glioma

  • Laboratory Investigation - Human/Animal Tissue
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Abstract

In this study, we demonstrate that tumor-derived exosome-loaded dendritic cells can elicit a specific CD8+ cytotoxic T-lymphocyte (CTL) response against autologous tumor cells in patients with malignant glioma. Exosomes were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation. Exosomes had antigen-presenting molecules (MHC-I, HSP70), tumor antigen (MAGE-1) and adherent molecule (ICAM-1). After incubation with exosomes, the dendritic cells (DCs) could activate the T lymphocytes to become glioma-specialized CTL. The CTL had vigorous cytotoxicity to glioma cells as opposed to autologous lymphoblast cells. These data demonstrate that tumor exosome-loaded DC can be an effective tool in inducing glioma-specific CD8+ CTLs able to kill autologous glioma cells in vitro. In conclusion, exosomes are a natural and new source of tumor-rejection antigens, opening up new avenues for immunization against glioma.

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Acknowledgments

This study was supported by the Xi’an Jiaotong University President’s Fund, no. xjj2008021. Contributions: Ning Bu obtained research funding, interpreted and analysed the data, and provided a draft of the article; Bingzhong Sun contributed to the concept and design, and provided critical revisions and important intellectual content; Shuqin Zhan, Ru Zhang, Le Zhou provided the study materials and administrative support; Haiqin Wu, Guilian Zhang, provided technical and logistical support.

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Authors and Affiliations

  1. Department of Neurology, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi, China

    Ning Bu, Haiqin Wu, Guilian Zhang, Shuqin Zhan & Ru Zhang

  2. Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, China

    Bingzhong Sun

  3. Department of Neurosurgery, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi, China

    Le Zhou

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  1. Ning Bu
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  2. Haiqin Wu
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Correspondence to Ning Bu.

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Bu, N., Wu, H., Sun, B. et al. Exosome-loaded dendritic cells elicit tumor-specific CD8+ cytotoxic T cells in patients with glioma. J Neurooncol 104, 659–667 (2011). https://doi.org/10.1007/s11060-011-0537-1

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  • DOI: https://doi.org/10.1007/s11060-011-0537-1

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