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Cellular DNA content parameters as prognostic indicators in human astrocytomas

  • Laboratory Investigation
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Abstract

Objective: Clinical parameters such as grade, size and/or location of the tumor are good predictors of outcome in patients with astrocytoma. The objective of this study was to determine whether DNA content parameters have a prognostic significance for this group of tumors.

Methods: Following optimization and validation of methodology for evaluating cellular DNA content parameters (CDCP), tumor DNA ploidy and percent S phase fraction (SPF) were determined from 64 patients using formalin fixed, paraffin embedded specimens (mean coefficient of variation = 4.94) obtained over a 10-year period. Median survival times correlated with grade (I/II =1154 vs. III/IV= 483days, P=0.0317). Fifty-five percent of the specimens contained DNA aneuploid (DNA-A) components (average SPF=18.3%) and 45% were DNA diploid (DNA-D) (average SPF=9.6%). Survival did not correlate with overall differences in DNA ploidy (DNA-D=181 vs. DNA-A=206days, P=0.6314) when treated and untreated tumors were analyzed. However, a trend for prolonged median survival was observed in patients whose tumors were untreated with respect to cytotoxic therapy based on DNA ploidy status (DNA-D=275 vs. DNA-A=15days, P=0.3408). Survival for all patients did not correlate with median SPF (< 13.5% av.= 121 vs. > 13.5% av.= 154days, P=0.6534).

Conclusion: DNA content parameters may correlate with the natural history and treatment outcome of newly diagnosed untreated patients with astrocytomas.

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Authors and Affiliations

  1. Department of Internal Medicine, Division of Hematology and Oncology, Wayne State University, Karmanos Cancer Institute, MI, USA

    Basil F. El-Rayes, Camille S. Norton, Daryn Smith, Haline Pietraszkiewicz, Maria Del Mar Alonso & John F. Ensley

  2. Department of Pathology, School of Medicine, Wayne State University, Karmanos Cancer Institute, Detroit, MI, USA

    Wael Sakr

  3. Curie Institute, Paris, France

    Zosia Maciorowski

  4. Harper Hospital, 3990 John R, Detroit, MI, 48201, USA

    John F. Ensley

Authors
  1. Basil F. El-Rayes
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  2. Camille S. Norton
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  3. Wael Sakr
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  4. Zosia Maciorowski
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  5. Daryn Smith
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  6. Haline Pietraszkiewicz
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  7. Maria Del Mar Alonso
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  8. John F. Ensley
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Corresponding author

Correspondence to John F. Ensley.

Additional information

Supported by NIH CA 40498–01A1; NIH P30CA22453

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El-Rayes, B.F., Norton, C.S., Sakr, W. et al. Cellular DNA content parameters as prognostic indicators in human astrocytomas. J Neurooncol 71, 85–89 (2005). https://doi.org/10.1007/s11060-004-6044-x

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