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Simple and efficient synthesis of copper(II)-modified uniform magnetic Fe3O4@SiO2 core/shell microspheres for immobilization of cellulase

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Abstract

In this paper, we reported a simple and efficient protocol for preparation of Cu2+-modified magnetic Fe3O4@SiO2 core/shell microspheres for immobilization of cellulase. The uniform magnetic Fe3O4@SiO2 core/shell microspheres with a thin shell of 20 nm were synthesized through a solvothermal method followed by a sol–gel process. An amino-terminated silane coupling agent of (3-aminopropyl)triethoxysilane (APTS) was then grafted on them for capturing Cu2+ ions. The reaction process is very simple, efficient, and economical. Noticeably, the content of Cu2+ ions on the magnetic core/shell microspheres can reach 4.6 Wt%, endowing them possess as high immobilization capacity as 225.5 mg/g for cellulase. And the immobilized cellulase can be retained over 90 % on the magnetic microspheres after six cycles. Meanwhile, the magnetic microspheres decorated with Cu2+ ions show a superparamagnetic character with a high magnetic saturation of 58.5 emu/g at room temperature, suggesting conveniently and rapidly recycle the enzyme from solution. This facile, recyclable, high immobilization capacity and activity strategy may find potential applications in enzyme catalytic reactions with low cost.

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Acknowledgments

This work is supported by the National Science Foundation of China (Grants 50973001, 21301001, 21303002), the Key Project of Anhui Provincial Education Department (KJ2011A021), Anhui Provincial Natural Science Foundation (1208085QB43, 1208085QB35, 11040606Q01), the Science Foundation for Excellent Youth Scholars of Higher Education of Anhui Province (2011SQRL016ZD), the Research Fund for the Doctoral Program of Higher Education of China (Ref. No.: 20123401120004), the Foundation of Key Laboratory of Environment-friendly Polymer Materials of Anhui Province (KF2012010).

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  1. School of Chemistry and Chemical Engineering, Anhui University, Hefei, 230039, People’s Republic China

    Shi-Kuo Li, Xiao-Cheng Hou, Fang-Zhi Huang, Chuan-Hao Li, Wen-Juan Kang, An-Jian Xie & Yu-Hua Shen

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  1. Shi-Kuo Li
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  2. Xiao-Cheng Hou
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Correspondence to An-Jian Xie or Yu-Hua Shen.

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Li, SK., Hou, XC., Huang, FZ. et al. Simple and efficient synthesis of copper(II)-modified uniform magnetic Fe3O4@SiO2 core/shell microspheres for immobilization of cellulase. J Nanopart Res 15, 2013 (2013). https://doi.org/10.1007/s11051-013-2013-7

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