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Evaluation of DNA damage and cytotoxicity of polyurethane-based nano- and microparticles as promising biomaterials for drug delivery systems

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Abstract

The in vitro cytotoxicity and DNA damage evaluation of biodegradable polyurethane-based micro- and nanoparticles were carried out on animal fibroblasts. For cytotoxicity measurement and primary DNA damage evaluation, MTT and Comet assays were used, respectively. Different formulations were tested to evaluate the influence of chemical composition and physicochemical characteristics of particles on cell toxicity. No inhibition of cells growth surrounding the polyurethane particles was observed. On the other hand, a decrease of cell viability was verified when the anionic surfactant sodium dodecyl sulfate (SDS) was used as droplets stabilizer of monomeric phase. Polyurethane nanoparticles stabilized with Tween 80 and Pluronic F68 caused minor cytotoxic effects. These results indicated that the surface charge plays an important role on cytotoxicity. Particles synthesized from MDI displayed a higher cytotoxicity than those synthesized from IPDI. Size and physicochemical properties of the particles may explain the higher degree of DNA damage produced by two tested formulations. In this way, a rational choice of particles’ constituents based on their cytotoxicity and genotoxicity could be very useful for conceiving biomaterials to be used as drug delivering systems.

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Acknowledgements

The Brazilian authors thank Capes/MEC and CNPq/MCT (Brazil) for their research fellowships.

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Authors and Affiliations

  1. Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, 88040-900, Brazil

    Thiago Caon & Cláudia Maria Oliveira Simões

  2. Laboratório de Estudos em Materiais Poliméricos, Departamento de Química, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, 88040-900, Brazil

    Betina Giehl Zanetti-Ramos & Valdir Soldi

  3. Laboratório de Farmacotécnica, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, 88040-900, Brazil

    Elenara Lemos-Senna

  4. Laboratoire de Chimie des Polymères Organiques (LCPO), CNRS/ENSCPB-Université Bordeaux 1 (UMR5629), 16 Avenue Pey-Berland, 33607, Pessac Cedex, France

    Eric Cloutet, Henri Cramail & Redouane Borsali

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  1. Thiago Caon
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  2. Betina Giehl Zanetti-Ramos
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  3. Elenara Lemos-Senna
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  4. Eric Cloutet
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  5. Henri Cramail
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  6. Redouane Borsali
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  7. Valdir Soldi
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  8. Cláudia Maria Oliveira Simões
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Correspondence to Cláudia Maria Oliveira Simões.

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Caon, T., Zanetti-Ramos, B.G., Lemos-Senna, E. et al. Evaluation of DNA damage and cytotoxicity of polyurethane-based nano- and microparticles as promising biomaterials for drug delivery systems. J Nanopart Res 12, 1655–1665 (2010). https://doi.org/10.1007/s11051-009-9828-2

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  • DOI: https://doi.org/10.1007/s11051-009-9828-2

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