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Could Helium Plasma Treatment be a Novel Approach to Prevent the Biofilm Formation of Candida albicans?

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Abstract

There is no definitive method to prevent Candida albicans (C. albicans) biofilm formation on polymethyl methacrylate (PMMA) surfaces. The objective of this study was to evaluate the effect of Helium plasma treatment (before the application of removable dentures to the patient) to prevent or reduce C. albicans ATCC 10,231 the anti-adherent activity, viability, and biofilm formation on PMMA surfaces. One hundred disc-shaped PMMA samples (2 mm × 10 mm) were prepared. The samples were randomly divided into 5 surface groups and treated with different concentrations of Helium plasma: G I: Control group (untreated), G II: 80% Helium plasma-treated group, G III: 85% Helium plasma-treated group, G IV: 90% Helium plasma-treated group, G V: 100% Helium plasma-treated group. C. albicans viability and biofilm formations were evaluated using 2 methods: MTT (3-(4,5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) assays and Crystal Violet (CV) staining. The surface morphology and C. albicans biofilm images were observed with scanning electron microscopy. The Helium plasma-treated PMMA groups (G II, G III, G IV, G V) observed a significant reduction in C. albicans cell viability and biofilm formation compared with the control group. Treating PMMA surfaces with different concentrations of Helium plasma prevents C. albicans viability and biofilm formation. This study suggests that Helium plasma treatment might be an effective strategy in modifying PMMA surfaces to prevent denture stomatitis formation.

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Data Availability

Data is available upon request from the corresponding author.

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Acknowledgements

This research was previosly presented as an oral presentation at the 9th International Academic Studies Conference, 3-5 August 2022 (Online). We thanks to ‘Advanced Material Technologies Application and Research Center’ for their support and contribution.

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Authors and Affiliations

  1. Department of Prosthodontics, Faculty of Dentistry, Osmangazi University, Eskişehir, Turkey

    Esra Nur Avukat, Canan Akay & Emre Mumcu

  2. Translational Medicine Research and Clinical Center, Osmangazi University, Eskisehir, Turkey

    Canan Akay & Suat Pat

  3. Advanced Material Technologies Application and Research Center, Osmangazi University, Eskisehir, Turkey

    Canan Akay, Emre Mumcu & Suat Pat

  4. Kütahya Oral and Dental Health Hospital, Kütahya, Turkey

    Mirac Berke Topcu Ersöz

  5. Department of Physics, Faculty of Science and Letters, Eskisehir Osmangazi University, Eskisehir, Turkey

    Suat Pat

  6. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Düzce University, Düzce, Turkey

    Demet Erdönmez

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  1. Esra Nur Avukat
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  2. Canan Akay
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  3. Mirac Berke Topcu Ersöz
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Correspondence to Esra Nur Avukat.

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Avukat, E.N., Akay, C., Topcu Ersöz, M.B. et al. Could Helium Plasma Treatment be a Novel Approach to Prevent the Biofilm Formation of Candida albicans?. Mycopathologia 188, 361–369 (2023). https://doi.org/10.1007/s11046-023-00747-9

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  • DOI: https://doi.org/10.1007/s11046-023-00747-9

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