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Pseudocanariomyces americanus, gen. nov., sp. nov., A New Thielavia-Like Species in the Chaetomiaceae: Identification and Management of a Prosthetic Hip Infection

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Abstract

This report describes the phenotypic characteristics of a novel fungal species, isolated from a prosthetic hip infection. The patient, who had undergone multiple total hip arthroplasties due to Legg-Calvé-Perthes disease, presented with continued fever and wound dehiscence. Findings upon incision and draining were notable for necrotic tissue and a sinus tract from the fluid collection. Intraoperative cultures were positive for a sterile filamentous fungus. BLASTn results following DNA sequencing placed the isolate within the family Chaetomiaceae close to the genera Madurella, Canariomyces, Stolonocarpus, Stellatospora, Ovatospora, Carteria and Melanocarpus. Phylogenetic analysis demonstrated that the isolate was a new thielavia-like species, Pseudocanariomyces americanus. Antifungal susceptibility was performed, and low minimum inhibitory concentrations were observed with amphotericin B, itraconazole, posaconazole, and voriconazole. The patient was initially treated with voriconazole but was switched to posaconazole secondary to a photosensitivity reaction. Acceptable posaconazole trough concentrations were achieved, and the patient remained stable without pain or drainage from her surgical incision.

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Data Availability

DNA sequences have been deposited into GenBank; other data are available upon request to the authors.

Materials Availability

Isolates are available upon reasonable request

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Authors and Affiliations

  1. Pharmacy Department, The University of New Mexico Hospital, Albuquerque, NM, USA

    Keenan Ryan

  2. Fungus Testing Laboratory, Department of Pathology and Laboratory Medicine, University of Texas Health Science Center At San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78228, USA

    Connie Cañete-Gibas, Carmita Sanders & Nathan P. Wiederhold

  3. Division of Infectious Diseases, Department of Internal Medicine, The University of New Mexico School of Medicine, Albuquerque, NM, USA

    Nestor Sosa

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  1. Keenan Ryan
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  2. Connie Cañete-Gibas
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  3. Carmita Sanders
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  4. Nestor Sosa
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  5. Nathan P. Wiederhold
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Correspondence to Nathan P. Wiederhold.

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Conflict of interest

N.P.W. has received grant support from Astellas, bioMerieux, Cepheid, and F2G, and has served on the scientific advisory boards for Mayne Pharma. All other authors report no conflicts.

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The authors confirm that the material presented in this manuscript is original and has not been published elsewhere, in whole or in part, and that all authors reviewed and approved the final version. In addition, the authors confirm completion of the checklist for submission of a case report to Mycopathologia, as published by Bouchara et al. [17].

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Supplemental Figure 1.

Maximum likelihood tree based on individual phylogenies of ITS, TUB2 and RPB2 nucleotide sequences of Pseudocanariomyces americanus and related taxa in the Chaetomiaceae. Posterior probabilities (> 0.95, left) and bootstrap values (80-100%, right) are shown on the nodes. T= type strain. (TIF 406 KB)

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Ryan, K., Cañete-Gibas, C., Sanders, C. et al. Pseudocanariomyces americanus, gen. nov., sp. nov., A New Thielavia-Like Species in the Chaetomiaceae: Identification and Management of a Prosthetic Hip Infection. Mycopathologia 186, 441–447 (2021). https://doi.org/10.1007/s11046-021-00555-z

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  • DOI: https://doi.org/10.1007/s11046-021-00555-z

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