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Molecular Epidemiology of Malassezia globosa and Malassezia restricta in Sudanese Patients with Pityriasis Versicolor

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Abstract

Pityriasis versicolor is a superficial infection of the stratum corneum caused by Malassezia yeasts. The cutaneous Malassezia globosa and Malassezia restricta in Sudanese patients with pityriasis versicolor were elucidated using a molecular-based, culture-independent method and compared with that in healthy individuals. Scale samples were collected by applying an Opsite™ transparent dressing to lesional and non-lesional sites on 29 Sudanese patients with pityriasis versicolor and 54 healthy individuals. Malassezia DNA was extracted directly from the samples. The overall level of colonization by Malassezia globosa and Malassezia restricta was analyzed by real-time PCR using a TaqMan probe. The overall level of colonization by Malassezia at the lesional sites was higher than that at the non-lesional sites for all body sites, including the face, neck, cheeks, and trunk (2.7- to 6.0-fold increase). Both M. globosa and M. restricta were detected in patients and healthy individuals. However, M. globosa predominated at lesional sites, whereas the level of colonization by both species was similar in healthy individuals.

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Acknowledgments

This study was supported in part by a Grant-in-Aid from the Japan Society for the Promotion of Science (JSPS), a research grant from the Asian and African Science Platform Program from JSPS, and the “High-Tech Research Center Project” from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (TS).

Conflict of interest

There were no conflicts of interest.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan

    M. Saad & H. Saeed

  2. Department of Microbiology, Meiji Pharmaceutical University, Tokyo, Japan

    T. Sugita

  3. Department Microbiology, College of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia

    A. Ahmed

Authors
  1. M. Saad
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  2. T. Sugita
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  3. H. Saeed
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  4. A. Ahmed
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Correspondence to M. Saad.

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Saad, M., Sugita, T., Saeed, H. et al. Molecular Epidemiology of Malassezia globosa and Malassezia restricta in Sudanese Patients with Pityriasis Versicolor. Mycopathologia 175, 69–74 (2013). https://doi.org/10.1007/s11046-012-9587-y

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  • DOI: https://doi.org/10.1007/s11046-012-9587-y

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