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A review of advances in aptamer-based cell detection technology

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Abstract

Since cells are the basic structural and functional units of organisms, the detection or quantitation of cells is one of the most common basic problems in life science research. The established cell detection techniques mainly include fluorescent dye labeling, colorimetric assay, and lateral flow assay, all of which employ antibodies as cell recognition elements. However, the widespread application of the established methods generally dependent on antibodies is limited, because the preparation of antibodies is complicated and time-consuming, and unrecoverable denaturation is prone to occur with antibodies. By contrast, aptamers that are generally selected through the systematic evolution of ligands by exponential enrichment can avoid the disadvantages of antibodies due to their controllable synthesis, thermostability, and long shelf life, etc. Accordingly, aptamers may serve as novel molecular recognition elements like antibodies in combination with various techniques for cell detection. This paper reviews the developed aptamer-based cell detection methods, mainly including aptamer-fluorescent labeling, aptamer-isothermal amplification assay, electrochemical aptamer sensor, aptamer-based lateral flow analysis, and aptamer-colorimetric assay. The principles, advantages, progress of application in cell detection and future development trend of these methods were specially discussed. Overall, different assays are suitable for different detection purposes, and the development of more accurate, economical, efficient, and rapid aptamer-based cell detection methods is always on the road in the future. This review is expected to provide a reference for achieving efficient and accurate detection of cells as well as improving the usefulness of aptamers in the field of analytical applications.

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Data Availability

All data are available from the authors upon reasonable request.

Abbreviations

RNA:

Ribonucleic acid

DNA:

Deoxyribonucleic acid

SELEX:

Systematic evolution of ligands by exponential enrichment

WC-SELEX:

Whole-cell SELEX

PCR:

Polymerase chain reaction

LIGS:

Ligand-guided selection

mAb:

Monoclonal antibody

mIgM:

Membrane-bound immunoglobulin

ssDNA:

Single-stranded DNA

HPLC:

High-performance liquid chromatography

PH:

Potential of hydrogen

LAMP:

Loop-mediated isothermal amplification

RCA:

Rolling circle amplification

HCR:

Hybridization chain reaction

L. monocytogenes :

Listeria monocytogenes

PLP:

Padlock probe

DNTPs:

Deoxynucleoside triphosphates

cDNA:

Complementary deoxyribonucleic acid

V. parahaemolyticus :

Vibrio Parahaemolyticus

IC:

Initiating chain

HP:

Hairpin probes

Aptasensors:

Aptamer sensors

E. coli :

Escherichia coli

CFU:

Colony forming units

LFA:

Lateral flow assay

GC:

Gas chromatography

GC-MS:

Gas chromatography-mass spectrometry

LC-MS:

Liquid chromatography-mass spectrometry

V. fischeri :

Vibrio fischeri

ATP:

Adenosine triphosphate

AuNPs:

Amphiphilic gold nanoparticles

GNPs:

Gold nanoparticles

SPR:

Surface plasmon resonanceATO

UV:

Ultraviolet

S. typhimurium :

Salmonella typhimurium

S. aureus :

Staphylococcus aureus

dsDNA:

Double-stranded DNA

SGI:

SYBR Green I

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Funding

This work was supported by Shandong Provincial Natural Science Foundation, China (ZR2020MD081); the National Scientific Foundation of China (No. 31600309, 41476086); HIT Scientific Research Innovation Fund (No. 2022KYCXJJ07); and HIT Environment and Ecology Innovation Special Funds (No. HSCJ201622).

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Authors and Affiliations

  1. School of Marine Science and Technology, Harbin Institute of Technology (Weihai), Wenhua West Road, 2#, Weihai, 264209, PR China

    Wenrong Chen, Fuguo Liu, Chunyun Zhang, Yu Duan, Jinju Ma, Yuanyuan Wang & Guofu Chen

  2. School of Environment, Harbin Institute of Technology, Harbin, 150090, PR China

    Fuguo Liu, Yu Duan & Jinju Ma

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  1. Wenrong Chen
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Conceived and designed the paper: Fuguo Liu, Chunyun Zhang and Guofu Chen; Analysis and arrangement of documents: Yu Duan, Jinju Ma, Yuanyuan Wang and Wenrong Chen; Wrote the paper: Wenrong Chen.

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Correspondence to Chunyun Zhang or Guofu Chen.

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Chen, W., Liu, F., Zhang, C. et al. A review of advances in aptamer-based cell detection technology. Mol Biol Rep 50, 5425–5438 (2023). https://doi.org/10.1007/s11033-023-08410-8

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