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Overexpression of Zostera japonica heat shock protein gene ZjHsp70 enhances the thermotolerance of transgenic Arabidopsis

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Abstract

Background

Heat shock protein 70s (Hsp70s) are major members of the heat shock protein family and play a variety of roles to protect plants against stress. Plant Hsp70s are a conserved and widely expressed family of heat shock proteins. They have two main functional regions: N-terminal nucleic acid binding region and C-terminal substrate binding region.

Methods and results

In this study, we cloned the Hsp70 gene of Zostera japonica (ZjHsp70) based on the sequence obtained by transcriptome sequencing. The transcriptional levels of ZjHsp70 increased significantly at 1 h after heat treatment. ZjHsp70 was located in the cytoplasm and nucleus. The overexpression of ZjHsp70 in Arabidopsis resulted in increased heat tolerance, lower contents of malondialdehyde and higher antioxidant enzyme activity than in the wild type. ZjHsp70 may achieve this goal by maintaining highly active antioxidant enzymes.

Conclusions

We show that ZjHsp70 can improve plant heat tolerance by maintaining high antioxidant enzyme activity under high temperature stress. This study provided a basis to study the role of ZjHsp70 in thermotolerance in more detail.

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Data availability

All data generated or analyzed during this study are included in this published article and its Supplementary Information files. The nucleotide and deduced amino acid sequence data of ZjHsp70 were registered in GenBank (Accession No. MK359368).

Code availability

Not applicable.

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Acknowledgements

This study was funded by the Natural Science Foundation of Guangxi Province (2020GXNSFAA297067; 2018GXNSFBA281062), the Research Fund Program of Guangxi Key Lab of Mangrove Conservation and Utilization (GKLMC-21A01; GKLMC-20A04; GKLMC-20A01), the National Natural Science Foundation of China (32170399) and the National Science & Technology Fundamental Resources Investigation Program of China (2019FY100604). These funding bodies had no role in the design of the study, collection, analysis, and interpretation of data, or in the writing of the manuscript.

Funding

This study was funded by the Natural Science Foundation of Guangxi Province (2020GXNSFAA297067; 2018GXNSFBA281062), the Research Fund Program of Guangxi Key Lab of Mangrove Conservation and Utilization (GKLMC-21A01; GKLMC-20A04; GKLMC-20A01), the National Natural Science Foundation of China (32170399) and the National Science & Technology Fundamental Resources Investigation Program of China (2019FY100604). These funding bodies had no role in the design of the study, collection, analysis, and interpretation of data, or in the writing of the manuscript.

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Authors and Affiliations

  1. Guangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai, 536007, Guangxi, China

    Siting Chen & Guanglong Qiu

Authors
  1. Siting Chen
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  2. Guanglong Qiu
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Contributions

SC designed the study and conducted the experiments. GQ conducted field sampling and identification. SC and GQ wrote the manuscript. All the authors reviewed the manuscript.

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Correspondence to Siting Chen or Guanglong Qiu.

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Chen, S., Qiu, G. Overexpression of Zostera japonica heat shock protein gene ZjHsp70 enhances the thermotolerance of transgenic Arabidopsis. Mol Biol Rep 49, 6189–6197 (2022). https://doi.org/10.1007/s11033-022-07411-3

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