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Effect of biodentine coated with emdogain on proliferation and differentiation of human stem cells from the apical papilla

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Abstract

Background

This study assessed the effect of Biodentine coated with Emdogain (Biodentine/Emdogain) on proliferation and differentiation of human stem cells from the apical papilla (SCAPs).

Methods and results

In this in vitro, experimental study, SCAPs were isolated from two immature impacted third molars and cultured. After ensuring the stemness of the cells by assessing the cell surface markers, they were exposed to Biodentine, Emdogain, and Biodentine/Emdogain for 24 and 72 h. The control cells did not receive any intervention. Cell viability was evaluated by the methyl thiazolyl tetrazolium assay. Expression of odontogenic differentiation genes was analyzed by the quantitative reverse transcription polymerase chain reaction. Alkaline phosphatase (ALP) activity was quantified by the respective kit. Data were analyzed by one-way ANOVA, t-test, and Mann-Whitney test (α = 0.05). Cell viability did not change after 24 h of exposure to biomaterials. At 72 h, the viability of the cells exposed to Biodentine and Biodentine/Emdogain decreased compared with the control group. The expression of dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein genes, and ALP activity significantly increased in all three experimental groups, compared with the control group at both 24 and 72 h; this increase was significantly greater in Biodentine/Emdogain group. The number of mineralized nodules significantly increased in all groups after 72 h with a greater rate in Biodentine/Emdogain group.

Conclusions

All biomaterials increased the differentiation of SCAPs, expression of odontogenic genes, and ALP activity, but Biodentine/Emdogain was significantly more effective for this purpose.

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References

  1. Diogenes A, Ruparel NB, Shiloah Y, Hargreaves KM (2016) Regenerative endodontics: a way forward. J Am Dent Assoc 147:372–380. https://doi.org/10.1016/j.adaj.2016.01.009

    Article  PubMed  Google Scholar 

  2. Chrepa V, Henry MA, Daniel BJ, Diogenes A (2015) Delivery of apical mesenchymal stem cells into root canals of mature teeth. J Dent Res 94:1653–1659. https://doi.org/10.1177/0022034515596527

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Chrepa V, Pitcher B, Henry MA, Diogenes A (2017) Survival of the apical papilla and its resident stem cells in a case of advanced pulpal necrosis and apical periodontitis. J Endod 43:561–567. https://doi.org/10.1016/j.joen.2016.09.024

    Article  PubMed  Google Scholar 

  4. Tobias Duarte PC, Gomes-Filho JE, Ervolino E, Marçal Mazza Sundefeld ML, Tadahirowayama M, Lodi CS, Dezan-Júnior E, Angelo Cintra LT (2014) Histopathological condition of the remaining tissues after endodontic infection of rat immature teeth. J Endod 40:538–542. https://doi.org/10.1016/j.joen.2013.09.015

    Article  PubMed  Google Scholar 

  5. Parirokh M, Torabinejad M (2010) Mineral trioxide aggregate: a comprehensive literature review—part III: clinical applications, drawbacks, and mechanism of action. J Endod 36:400–413. https://doi.org/10.1016/j.joen.2009.09.009

    Article  PubMed  Google Scholar 

  6. Kim Y, Lee D, Kim HM, Kye M, Kim SY (2021) Biological characteristics and odontogenic differentiation effects of calcium silicate-based pulp capping materials. Materials (Basel) 14:4661

    Article  CAS  Google Scholar 

  7. Jitaru S, Hodisan I, Timis L, Lucian A, Bud M (2016) The use of bioceramics in endodontics—literature review. Clujul Med 89:470–473. https://doi.org/10.15386/cjmed-612

    Article  PubMed  PubMed Central  Google Scholar 

  8. Haglund R, He J, Jarvis J, Safavi KE, Spångberg LS, Zhu Q (2003) Effects of root-end filling materials on fibroblasts and macrophages in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 95:739–745. https://doi.org/10.1067/moe.2003.231

    Article  PubMed  Google Scholar 

  9. Edrees HY, Abu Zeid STH, Atta HM, AlQriqri MA (2019) Induction of osteogenic differentiation of mesenchymal stem cells by bioceramic root repair material. Materials (Basel) 12:2311

    Article  CAS  Google Scholar 

  10. Lee BN, Moon JW, Chang HS, Hwang IN, Oh WM, Hwang YC (2015) A review of the regenerative endodontic treatment procedure. Restor Dent Endod 40:179–187. https://doi.org/10.5395/rde.2015.40.3.179

    Article  PubMed  PubMed Central  Google Scholar 

  11. Goyal L (2014) Clinical effectiveness of combining platelet rich fibrin with alloplastic bone substitute for the management of combined endodontic periodontal lesion. Restor Dent Endod 39:51–55. https://doi.org/10.5395/rde.2014.39.1.51

    Article  PubMed  PubMed Central  Google Scholar 

  12. Hotwani K, Sharma K (2014) Platelet rich fibrin—a novel acumen into regenerative endodontic therapy. Restor Dent Endod 39:1–6. https://doi.org/10.5395/rde.2014.39.1.1

    Article  PubMed  PubMed Central  Google Scholar 

  13. Bonson S, Jeansonne BG, Lallier TE (2004) Root-end filling materials alter fibroblast differentiation. J Dent Res 83:408–413. https://doi.org/10.1177/154405910408300511

    Article  CAS  PubMed  Google Scholar 

  14. Witherspoon DE, Small JC, Regan JD, Nunn M (2008) Retrospective analysis of open apex teeth obturated with mineral trioxide aggregate. J Endod 34:1171–1176. https://doi.org/10.1016/j.joen.2008.07.005

    Article  PubMed  Google Scholar 

  15. Camilleri J (2008) Characterization of hydration products of mineral trioxide aggregate. Int Endod J 41:408–417. https://doi.org/10.1111/j.1365-2591.2007.01370.x

    Article  CAS  PubMed  Google Scholar 

  16. Camilleri J, Kralj P, Veber M, Sinagra E (2012) Characterization and analyses of acid-extractable and leached trace elements in dental cements. Int Endod J 45:737–743. https://doi.org/10.1111/j.1365-2591.2012.02027.x

    Article  CAS  PubMed  Google Scholar 

  17. Han J, Menicanin D, Gronthos S, Bartold PM (2014) Stem cells, tissue engineering and periodontal regeneration. Aust Dent J 59:117–130. https://doi.org/10.1111/adj.12100

    Article  PubMed  Google Scholar 

  18. Escobar-García DM, Aguirre-López E, Méndez-González V, Pozos-Guillén A (2016) Cytotoxicity and initial biocompatibility of endodontic biomaterials (MTA and Biodentine™) used as root-end filling materials. Biomed Res Int 2016:7926961. https://doi.org/10.1155/2016/7926961

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Miron RJ, Caluseru OM, Guillemette V, Zhang Y, Gemperli AC, Chandad F, Sculean A (2013) Influence of enamel matrix derivative on cells at different maturation stages of differentiation. PLoS ONE 8:e71008. https://doi.org/10.1371/journal.pone.0071008

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Frasheri I, Ern C, Diegritz C, Hickel R, Hristov M, Folwaczny M (2016) Full-length amelogenin influences the differentiation of human dental pulp stem cells. Stem Cell Res Ther 7:10. https://doi.org/10.1186/s13287-015-0269-9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Gambarini G, Plotino G, Grande NM, Nocca G, Lupi A, Giardina B, De Luca M, Testarelli L (2011) In vitro evaluation of the cytotoxicity of FotoSan™ light-activated disinfection on human fibroblasts. Med Sci Monit 17:MT21–MT25. https://doi.org/10.12659/msm.881435

    Article  PubMed  PubMed Central  Google Scholar 

  22. Bakopoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, Geurtsen W (2011) Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP). Arch Oral Biol 56:709–721. https://doi.org/10.1016/j.archoralbio.2010.12.008

    Article  CAS  PubMed  Google Scholar 

  23. Wu J, Jia Q, He W, Liu J, Hou L, Zhang J, Niu Z, Ni L (2013) Conditioned medium from periapical follicle cells induces the odontogenic differentiation of stem cells from the apical papilla in vitro. J Endod 39:1015–1022. https://doi.org/10.1016/j.joen.2013.04.011

    Article  PubMed  Google Scholar 

  24. Pereira LO, Longo JP, Azevedo RB (2012) Laser irradiation did not increase the proliferation or the differentiation of stem cells from normal and inflamed dental pulp. Arch Oral Biol 57:1079–1085. https://doi.org/10.1016/j.archoralbio.2012.02.012

    Article  PubMed  Google Scholar 

  25. Paranjpe A, Zhang H, Johnson JD (2010) Effects of mineral trioxide aggregate on human dental pulp cells after pulp-capping procedures. J Endod 36:1042–1047. https://doi.org/10.1016/j.joen.2010.02.013

    Article  PubMed  Google Scholar 

  26. Hatakeyama J, Philp D, Hatakeyama Y, Haruyama N, Shum L, Aragon MA, Yuan Z, Gibson CW, Sreenath T, Kleinman HK, Kulkarni AB (2006) Amelogenin-mediated regulation of osteoclastogenesis, and periodontal cell proliferation and migration. J Dent Res 85:144–149. https://doi.org/10.1177/154405910608500206

    Article  CAS  PubMed  Google Scholar 

  27. Wang Y, Zhao Y, Ge L (2014) Effects of the enamel matrix derivative on the proliferation and odontogenic differentiation of human dental pulp cells. J Dent 42:53–59. https://doi.org/10.1016/j.jdent.2013.10.020

    Article  CAS  PubMed  Google Scholar 

  28. Paduano F, Marrelli M, Amantea M, Rengo C, Rengo S, Goldberg M, Spagnuolo G, Tatullo M (2017) Adipose tissue as a strategic source of mesenchymal stem cells in bone regeneration: a topical review on the most promising craniomaxillofacial applications. Int J Mol Sci 18:2140. https://doi.org/10.3390/ijms18102140

    Article  CAS  PubMed Central  Google Scholar 

  29. Karkehabadi H, Shahriari S, Najafi R, Khoshbin E, Abbaspourrokni H, Pakseresht Z (2019) Effect of Emdogain coated endodontic materials on viability of human dental pulp stem cells (HDPSCs). Giornale Italiano di Endodonzia 33.

  30. Saberi EA, Karkehabadi H, Mollashahi NF (2016) Cytotoxicity of various endodontic materials on stem cells of human apical papilla. Iran Endod J 11:17–22. https://doi.org/10.7508/iej.2016.01.004

    Article  CAS  PubMed  Google Scholar 

  31. Mohamed DA, Fayyad DM (2017) The effect of different bioactive materials on the odontogenic differentiation potential of dental pulp stem cells using two different culture mediums. Tanta Dental J 14:120–128

    Article  Google Scholar 

  32. Hwang YC, Hwang IN, Oh WM, Park JC, Lee DS, Son HH (2008) Influence of TGF-beta1 on the expression of BSP, DSP, TGF-beta1 receptor I and Smad proteins during reparative dentinogenesis. J Mol Histol 39:153–160. https://doi.org/10.1007/s10735-007-9148-8

    Article  CAS  PubMed  Google Scholar 

  33. Carnio J, Camargo PM, Kenney EB, Schenk RK (2002) Histological evaluation of 4 cases of root coverage following a connective tissue graft combined with an enamel matrix derivative preparation. J Periodontol 73:1534–1543. https://doi.org/10.1902/jop.2002.73.12.1534

    Article  PubMed  Google Scholar 

  34. Hammarström L, Heijl L, Gestrelius S (1997) Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins. J Clin Periodontol 24:669–677. https://doi.org/10.1111/j.1600-051x.1997.tb00248.x

    Article  PubMed  Google Scholar 

  35. Castellanos A, de la Rosa M, de la Garza M, Caffesse RG (2006) Enamel matrix derivative and coronal flaps to cover marginal tissue recessions. J Periodontol 77:7–14. https://doi.org/10.1902/jop.2006.77.1.7

    Article  PubMed  Google Scholar 

  36. Wu SM, Chiu HC, Chin YT, Lin HY, Chiang CY, Tu HP, Fu MM, Fu E (2014) Effects of enamel matrix derivative on the proliferation and osteogenic differentiation of human gingival mesenchymal stem cells. Stem Cell Res Ther 5:52. https://doi.org/10.1186/scrt441

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Li G, Hu J, Chen H, Chen L, Zhang N, Zhao L, Wen N, Yang Y (2017) Enamel matrix derivative enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells on the titanium implant surface. Organogenesis 13:103–113. https://doi.org/10.1080/15476278.2017.1331196

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Miller AA, Takimoto K, Wealleans J, Diogenes A (2018) Effect of 3 bioceramic materials on stem cells of the apical papilla proliferation and differentiation using a dentin disk model. J Endod 44:599–603. https://doi.org/10.1016/j.joen.2017.12.018

    Article  PubMed  Google Scholar 

  39. Min KS, Yang SH, Kim EC (2009) The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells. J Endod 35:847–851. https://doi.org/10.1016/j.joen.2009.03.014

    Article  PubMed  Google Scholar 

  40. Ching HS, Luddin N, Rahman IA, Ponnuraj KT (2017) Expression of odontogenic and osteogenic markers in DPSCs and SHED: a review. Curr Stem Cell Res Ther 12:71–79. https://doi.org/10.2174/1574888x11666160815095733

    Article  CAS  PubMed  Google Scholar 

  41. Jue SS, Lee WY, Kwon YD, Kim YR, Pae A, Lee B (2010) The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells. Clin Oral Implants Res 21:741–746. https://doi.org/10.1111/j.1600-0501.2009.01901.x

    Article  PubMed  Google Scholar 

  42. Papagerakis P, Berdal A, Mesbah M, Peuchmaur M, Malaval L, Nydegger J, Simmer J, Macdougall M (2002) Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth. Bone 30:377–385. https://doi.org/10.1016/s8756-3282(01)00683-4

    Article  CAS  PubMed  Google Scholar 

  43. Asgary S, Nazarian H, Khojasteh A, Shokouhinejad N (2014) Gene expression and cytokine release during odontogenic differentiation of human dental pulp stem cells induced by 2 endodontic biomaterials. J Endod 40:387–392. https://doi.org/10.1016/j.joen.2013.09.017

    Article  PubMed  Google Scholar 

  44. Chen CC, Shie MY, Ding SJ (2011) Human dental pulp cell responses to new calcium silicate-based endodontic materials. Int Endod J 44:836–842. https://doi.org/10.1111/j.1365-2591.2011.01890.x

    Article  CAS  PubMed  Google Scholar 

  45. Rathinam E, Rajasekharan S, Chitturi RT, Martens L, De Coster P (2015) Gene expression profiling and molecular signaling of dental pulp cells in response to tricalcium silicate cements: a systematic review. J Endod 41:1805–1817. https://doi.org/10.1016/j.joen.2015.07.015

    Article  PubMed  Google Scholar 

  46. Hajizadeh N, Madani ZS, Zabihi E, Golpour M, Zahedpasha A, Mohammadnia M (2018) Effect of MTA and CEM on mineralization-associated gene expression in stem cells derived from apical papilla. Iran Endod J 13:94–101. https://doi.org/10.22037/iej.v13i1.17860

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Saberi E, Farhad-Mollashahi N, Sargolzaei Aval F, Saberi M (2019) Proliferation, odontogenic/osteogenic differentiation, and cytokine production by human stem cells of the apical papilla induced by biomaterials: a comparative study. Clin Cosmet Investig Dent 11:181–193. https://doi.org/10.2147/CCIDE.S211893

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Mahapatra D, Nikhade DP, Sukhtankar DS, Padmanabha DP (2020) Enamel matrix derivative in pulpal regeneration and repair. Eur J Mol Clin Med 7:1731–1737

    Google Scholar 

  49. Iwata T, Morotome Y, Tanabe T, Fukae M, Ishikawa I, Oida S (2002) Noggin blocks osteoinductive activity of porcine enamel extracts. J Dent Res 81:387–391. https://doi.org/10.1177/0810387

    Article  CAS  PubMed  Google Scholar 

  50. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 97:13625–13630. https://doi.org/10.1073/pnas.240309797

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Funding

This study was financially supported by the School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran (Contract Number: 9811298972).

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Authors and Affiliations

  1. Department of Endodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran

    Hamed Karkehabadi, Erfan Ahmadyani & Elham Khoshbin

  2. Department of Molecular Medicine and Genetics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Rezvan Najafi

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  1. Hamed Karkehabadi
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  2. Erfan Ahmadyani
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  4. Elham Khoshbin
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Correspondence to Elham Khoshbin.

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Hamed Karkehabadi, Erfan Ahmadyani, Rezvan Najafi, Elham Khoshbin declare that they have no conflict of interest.

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All procedures performed in this study were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Karkehabadi, H., Ahmadyani, E., Najafi, R. et al. Effect of biodentine coated with emdogain on proliferation and differentiation of human stem cells from the apical papilla. Mol Biol Rep 49, 3685–3692 (2022). https://doi.org/10.1007/s11033-022-07208-4

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  • DOI: https://doi.org/10.1007/s11033-022-07208-4

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