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Platelet activation and antimicrobial activity of L-PRF: a preliminary study

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Abstract

Leukocyte and platelet rich fibrin (L-PRF) is one of the platelet concentrates used to support regeneration and healing process. Many studies showed possible immunological and antibacterial properties of L-PRF. We perform an in vitro study to analyze the effect of L-PRF on platelet activation, platelet-leukocytes interactions and antimicrobial activity, important components in the healing process. Molecular biomarkers related with platelet activation and platelet-leukocyte interactions were analyzed by means of flow cytometry when L-PRF exudate was added to whole blood platelets. L-PRF membrane was used to evaluate antimicrobial activity using Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 90028). Our experimental design allows to evaluate platelet activation and analyze molecular biomarkers of other immune cells and platelet-leukocyte interactions. From the results obtained we can conclude that L-PRF can be a valuable tool in healing process, efficient in activating platelets of whole blood and inhibiting microbial growth. In our opinion, the use of L-PRF exudate, in addition to L-PRF membrane, presents some advantages that have to be considered in clinical trials. Additional research on the characterization and quantification of cells and its products present in the L-PRF exudate, as well as on the temporal factor released. Also, further studies using strains isolated from clinical cases are needed.

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Acknowledgements

Authors thank the Institute of Research and Advance Training in Health Sciences and Technologies (IINFACTS-CESPU) for hosting the study.

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No funding was received for conducting this study.

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Authors and Affiliations

  1. IINFACTS – Institute of Research and Advanced Training in Health Sciences and Technology, CESPU-Cooperativa de Ensino Superior Politécnico e Universitário, Rua Central de Gandra 1317, 4585 116, Gandra, PRD, Portugal

    António Melo-Ferraz, Cristina Coelho, Paulo Miller, Maria Begoña Criado & Maria Céu Monteiro

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  1. António Melo-Ferraz
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  2. Cristina Coelho
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  3. Paulo Miller
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  4. Maria Begoña Criado
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Correspondence to Maria Céu Monteiro.

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Blood samples were collected with the informed consent of volunteer donors. All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional research committee and with Helsinki declaration and its later amendments. No ethical approval was required for this study because human samples were not identified.

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Melo-Ferraz, A., Coelho, C., Miller, P. et al. Platelet activation and antimicrobial activity of L-PRF: a preliminary study. Mol Biol Rep 48, 4573–4580 (2021). https://doi.org/10.1007/s11033-021-06487-7

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