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PPARGC1A gene polymorphism is associated with exercise-induced fat loss

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Abstract

Obesity is a widespread problem within modern society, serving to increase the risk of cardiovascular, metabolic, and neurodegenerative disorders. Peroxisome proliferator-activated receptor gamma (PPARγ) and PPARγ coactivator 1 α (PGC1α) play a key role in the regulation of cellular energy metabolism and is implicated in the pathology of these diseases. This study examined the association between polymorphisms of the PPARG and PPARGC1A genes and individual variability in weight loss in response to physical activity intervention. 39 obese Ukrainian women (44.4 ± 7.5 years, BMI > 30.0 kg/m2) undertook a 3-month fitness program whilst following a hypocaloric diet (~ 1500 cal). Anthropometric and biochemical measurements took place before and after the program. Single nucleotide polymorphisms within or near PPARG (n = 94) and PPARGC1A (n = 138) were identified and expression of PPARG mRNA was measured via reverse transcription and amplification. The association between DNA polymorphisms and exercise-induced weight loss, initial body mass, biochemistry and PPARG expression was determined using one-way analysis of variance (ANOVA). The present intervention induced significant fat loss in all participants (total fat: 40.3 ± 5.3 vs 36.4 ± 5.7%; P < 0.00001). Only one polymorphism (rs17650401 C/T) within the PPARGC1A gene was found to be associated with fat loss efficiency after correction for multiple testing, with T allele carriers showing the greatest reduction in body fat percentage (2.5-fold; P = 0.00013) compared to non-carriers. PPARGC1A (rs17650401) is associated with fat loss efficiency of the fitness program in obese women. Further studies are warranted to test whether this variation is associated with fat oxidation.

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Authors and Affiliations

  1. Medical and Biology Department, National University of Physical Education and Sport of Ukraine, 1 Fizkul’tury st., Kyiv, 03150, Ukraine

    Iuliia Iu. Mazur, Svitlana Drozdovska, Olena Andrieieva, Yulia Vinnichuk & Anna Polishchuk

  2. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    Victor Dosenko

  3. Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    Igor Andreev

  4. Institute of Coaching and Performance, School of Sport and Wellbeing, University of Central Lancashire, Preston, UK

    Craig Pickering

  5. Laboratory of Molecular Genetics, Kazan State Medical University, Kazan, Russia

    Ildus I. Ahmetov

  6. Department of Physical Education, Plekhanov Russian University of Economics, Moscow, Russia

    Ildus I. Ahmetov

  7. Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UK

    Ildus I. Ahmetov

Authors
  1. Iuliia Iu. Mazur
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  2. Svitlana Drozdovska
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  3. Olena Andrieieva
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  4. Yulia Vinnichuk
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  5. Anna Polishchuk
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  6. Victor Dosenko
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  7. Igor Andreev
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  8. Craig Pickering
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  9. Ildus I. Ahmetov
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Contributions

IIM contributed to data collection, data analysis, interpretation of results and manuscript writing; SD participated in the design of the study, data analysis, interpretation of results and manuscript writing;OA, YV, AP and IA contributed to data collection, data analysis and interpretation of results; VD participated in the design of the study, data analysis and interpretation of results; CP participated in the interpretation of results and manuscript writing; IIA participated in the design of the study, contributed to data analysis, interpretation of results and manuscript writing. All authors have read and approved the final version of the manuscript, and agree with the order of presentation of the authors.

Corresponding author

Correspondence to Iuliia Iu. Mazur.

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Conflict of interest

CP is a former employee of DNAfit Life Sciences, a genetic testing company. The remaining authors declare no conflicts of interest. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Ethical approval

This study was approved by the National Academy of Science of Ukraine Bogomoletz Institute of Physiology Biomedical Ethics Committee, Kyiv, Ukraine (#1/18 05.04.2018). Written informed consent was obtained from the participants, and the study conformed to the guidelines and principles of the Declaration of Helsinki.

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Mazur, I.I., Drozdovska, S., Andrieieva, O. et al. PPARGC1A gene polymorphism is associated with exercise-induced fat loss. Mol Biol Rep 47, 7451–7457 (2020). https://doi.org/10.1007/s11033-020-05801-z

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  • DOI: https://doi.org/10.1007/s11033-020-05801-z

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