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Production of ascorbic acid, total protein, callus and root in vitro of non-heading Chinese cabbage by tissue culture

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Abstract

The objective of the present work was the selection of cultivar, suitable medium and explant type for callus, root production, ascorbic acid, total ascorbic acid, dehydroascorbic and total protein of non-heading Chinese cabbage in two cultivars ‘Caixin’ and ‘Suzhouqing’. We compared 10 types of MS media supplemented with 0.0, 1.0, 2.0 and 3.0 mg/l TDZ; 0.0, 0.25, 0.50 and 1.0 mg/l NAA and 0.0, 5.0, 7.5 and 9.0 mg/l AgNO3 and 5 kinds of explants as embryo, leaf, root, cotyledon and hypocotyl. Maximum frequency of callus fresh weight was recorded with hypocotyl explant, which were cultured on MS + 2.0 mg/l TDZ + 1.0 mg/l NAA + 9.0 mg/l AgNO3 in ‘Suzhouqing’, optimum callus dry weight was obtained on the same media. The highest result for root fresh and dry weight recorded with ‘Caixin’ with MS + 3.0 mg/l TDZ + 1.0 mg/l NAA + 9.0 mg/l AgNO3 when we used embryo as explant. The highest ascorbic acid content was found with callus cultured on MS + 1.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO3, when used leaf explant in ‘Caixin’ or root in ‘Suzhouqing’, and there were no significant difference between them. While the highest value of total AsA content was registered with callus cultured on MS + 2.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO3 extracted from cotyledon in ‘Caixin’. The highest content of DHA was registered with MS + 2.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO3 with cotyledon in ‘Caixin’. Also, in ‘Caixin’ MS + 3.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO3 recorded the highest value of total protein content with embryo explant.

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Abbreviations

CKs:

Cytokinins

TDZ:

Thidiazuron

NAA:

Naphthalene acetic acid

AgNO3 :

Silver nitrate

AsA:

Ascorbic acid

T (AsA):

Total ascorbic acid

DHA:

Dehydroascorbic acid

TP:

Total protein

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Acknowledgements

This work was supported by the National Key Research and Development Program (2018YFD1000800), the National Natural Science Foundation of China (31872106), and National vegetable industry technology system (CARS-23-A-06).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China

    Osama M. Kamal, Sayyed Hamad Ahmad Shah, Yan Li, Xilin Hou & Ying Li

  2. Horticultural Department, Nanjing Agricultural University, Nanjing, China

    Osama M. Kamal, Sayyed Hamad Ahmad Shah, Yan Li, Xilin Hou & Ying Li

  3. Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing, 210095, China

    Osama M. Kamal, Sayyed Hamad Ahmad Shah, Yan Li, Xilin Hou & Ying Li

  4. Horticulture Dept, Faculty of Agriculture, Al Azhar University, Cairo, Egypt

    Osama M. Kamal

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  1. Osama M. Kamal
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  3. Yan Li
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Correspondence to Ying Li.

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Kamal, O.M., Shah, S.H.A., Li, Y. et al. Production of ascorbic acid, total protein, callus and root in vitro of non-heading Chinese cabbage by tissue culture. Mol Biol Rep 47, 6887–6897 (2020). https://doi.org/10.1007/s11033-020-05745-4

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