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Advances of exosome isolation techniques in lung cancer

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Abstract

Lung cancer (LC) is among the leading causes of death all over the world and it is often diagnosed at advanced or metastatic stages. Exosomes, derived from circulating vesicles that are released from the multivesicular body, can be utilized for diagnosis and also the prognosis of LC at early stages. Exosomal proteins, RNAs, and DNAs can help to better discern the prognostic and diagnostic features of LC. To our knowledge, there are various reviews on LC and the contribution of exosomes, but none of them are about the exome techniques and also their efficiency in LC. To fill this gap, in this review, we summarize the recent investigations regarding isolation and also the characterization of exosomes of LC cells. Furthermore, we discuss the noncoding RNAs as biomarkers and their applications in the diagnosis and prognosis of LC. Finally, we compare the efficacy of exosome isolation methods to better fi + 6 + guring out feasible techniques.

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Acknowledgements

This article was made possible by the grant NPRP10-120-170-211 from Qatar national Research Fund (a part of Qatar Foundation).

Funding

This article was made possible by the NPRP10-0120-170211 as a grant which was funded by Qatar National Research Fund (a part of Qatar Foundation).

Author information

Authors and Affiliations

  1. Department of Science and Engineering, Hamad Bin Khalifa University, Qatar Foundation, Education City, Doha, Qatar

    Elham O. Mahgoub & Yousif Haik

  2. Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Ehsan Razmara

  3. Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box: 14115-154, Tehran, Iran

    Amirreza Bitaraf, Fahimeh-Sadat Norouzi & Sadegh Babashah

  4. Department of Medical Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Maryam Montazeri

  5. Department of Biology, Central Branch, Islamic Azad University, Tehran, Iran

    Roudabeh Behzadi-Andouhjerdi

  6. Department of Nanotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Mojtaba Falahati

  7. Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill, North Carolina State University, NC, Raleigh, USA

    Ke Cheng

  8. Department of Molecular Biomedical Sciences and Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA

    Ke Cheng

  9. Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, 2713, Doha, Qatar

    Anwarul Hasan

  10. Biomedical Research Center, Qatar University, 2713, Doha, Qatar

    Anwarul Hasan

Authors
  1. Elham O. Mahgoub
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  2. Ehsan Razmara
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  3. Amirreza Bitaraf
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  4. Fahimeh-Sadat Norouzi
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  5. Maryam Montazeri
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  6. Roudabeh Behzadi-Andouhjerdi
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  7. Mojtaba Falahati
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  8. Ke Cheng
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  9. Yousif Haik
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  10. Anwarul Hasan
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  11. Sadegh Babashah
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Contributions

Conceptualization: Elham O. Mahgoub, Sadegh Babashah, Ehsan Razmara, and Anwarul Hasan; methodology: Ke Cheng, Fahimeh-Sadat Norouzi, and Mojtaba Falahati; Formal analysis and investigation: Roudabeh Behzadi-Andouhjerdi and Maryam Montazeri; writing-original draft preparation: Ehsan Razmara; writing—review and editing: Yousif Haik, Sadegh Babashah, and Amirreza Bitaraf; funding acquisition: Anwarul Hasan.

Corresponding authors

Correspondence to Anwarul Hasan or Sadegh Babashah.

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Mahgoub, E.O., Razmara, E., Bitaraf, A. et al. Advances of exosome isolation techniques in lung cancer. Mol Biol Rep 47, 7229–7251 (2020). https://doi.org/10.1007/s11033-020-05715-w

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  • DOI: https://doi.org/10.1007/s11033-020-05715-w

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