Abstract
Ten microsatellite markers were developed using next-generation sequencing data for Alibertia edulis (Rubiaceae), a widely distributed species typical of Cerrado (Brazilian savanna) vegetation. The markers were polymorphic in the two populations analyzed. The numbers of alleles, and observed (HO) and expected (HE) heterozygosities per polymorphic locus ranged from 2 to 11, 0.091 to 1.0, and 0.100 to 0.937 respectively. The SSR loci demonstrated moderate to high polymorphism values in both populations analyzed, with PIC values ranging from 0.26 to 0.91, and total allele numbers ranging from three to 16. The inbreeding coefficient values were generally higher in the Piauí population (ranging from − 0.593 to 0.762) than in the Mato Grosso population (ranging from − 1 to 0.575). The differences observed between those disjunct populations suggest they harbor different alleles, which has implications for Cerrado conservation strategies. Those loci will be useful for population studies of A. edulis.
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Acknowledgements
We thank Ana Paula Moraes, Felipe Amorim and the Conservation Area managers for their support in the field, and Ana Benko-Iseppon and João Pacífico Bezerra Neto for their assistance with the NGS data analysis. We also thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—fellowship 140699/2010-4), the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE APQ-0862-2.05/10), and the Fundação Grupo Boticário de Proteção à Natureza (Grant No. 0920_20112) for financial support. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. The authors declare that they have no conflicts of interest.
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Dantas, L.G., Alencar, L., Huettel, B. et al. Development of ten microsatellite markers for Alibertia edulis (Rubiaceae), a Brazilian savanna tree species. Mol Biol Rep 46, 4593–4597 (2019). https://doi.org/10.1007/s11033-019-04819-2
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DOI: https://doi.org/10.1007/s11033-019-04819-2