Abstract
Development of aluminium (Al) resistant genotypes through molecular breeding is a major approach for increasing seed yield under acidic conditions. There are no available reports on mapping of Al resistance loci and molecular breeding for Al resistant varieties in lentil. The present study reports a major quantitative trait loci (QTL) for Al resistance using simple sequence repeat (SSR) markers in F2 and F3 mapping populations derived from contrasting parents. Phenotypic response to Al was measured on the bases of root re-growth (RRG), fluorescent signals (callose accumulation) and Al contents in hydroponic assay. After screening 495 SSR markers to search polymorphism between two contrasting parents, 73 polymorphic markers were used for bulk segregation analysis. Two major QTLs were identified using seven trait linked markers, one each for fluorescent signals and RRG mapped on linkage group (LG) 1 under Al stress conditions in F2 mapping population of cross BM-4 × L-4602. One major QTL (qAlt_fs) was localised between PLC_88 and PBA_LC_373, covering 25.9 cM with adjacent marker PLC_88 at a distance of 0.4 cM. Another major QTL (qAlt_rrg) for RRG was in the marker interval of PBA_LC_1247 and PLC_51, covering a distance of 45.7 cM with nearest marker PBA_LC_1247 at a distance of 21.2 cM. Similarly, in F3 families of BM-4 × L-4602 and BM-4 × L-7903, LG-1 was extended to 285.9 and 216.4 cM respectively, having four newly developed genic-SSR markers. These QTLs had a logarithm of odd (LOD) value of 140.5 and 28.8 along with phenotypic variation of 52% and 11% for fluorescent signals and RRG respectively, whereas, qAlt_rrg had LOD of 36 and phenotypic variance of 25% in F3 population of BM-4 × L-4602. Two major QTLs identified in the present study can be further dissected for candidate gene discovery and development of molecular markers for breeding improved varieties with high Al resistance.
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Acknowledgements
Authors thank to Director, Joint Director (Res), ICAR-Indian Agricultural Research Institute (IARI), New Delhi, Head, Division of Genetics and Incharge, National Phytotron Facility, IARI, New Delhi, for given support to accomplish the research activities. The financial support from ICAR-IARI (Project no-JAN 09/16), New Delhi is gratefully acknowledged.
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Conceived and designed the experiments: DS, MP, KCU; Performed the experiments: CKS, SK; Analyzed the data: CKS, RSST; Drafted the manuscript: DS, CKS.
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Fig. S1
Determination of Al contents in roots of tolerant parent and tolerant F2 plants as well as sensitive parent and sensitive F2 plants. (TIF 10212 KB)
Fig. S2
Electrophoretic profile of PLC_88 in parents, bulks and selected F2 individuals. TP- Tolerant parent (L-4602), SP- sensitive parent (BM-4), TB- tolerant bulk, SB- Sensitive bulk, L- 100bp Ladder. (JPG 111 KB)
Fig. S3
Validation of Al linked marker, PLC_88 in Al resistant and sensitive genotypes. T- Tolerant parent, S- Sensitive parent of F2 population. (JPG 220 KB)
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Singh, C.K., Singh, D., Tomar, R.S.S. et al. Molecular mapping of aluminium resistance loci based on root re-growth and Al-induced fluorescent signals (callose accumulation) in lentil (Lens culinaris Medikus). Mol Biol Rep 45, 2103–2113 (2018). https://doi.org/10.1007/s11033-018-4368-4
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DOI: https://doi.org/10.1007/s11033-018-4368-4