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Characterization of Fusarium wilt resistant somaclonal variants of banana cv. Rasthali by cDNA-RAPD

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Abstract

Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense (Foc), is counted among the most destructive diseases of crop plants in India. In the absence of any credible control measure to manage this disease, development of resistant cultivars is the best option. Somaclonal variations arising out of long term in vitro culture of plant tissues is an important source of genetic variability and the selection of somaclones having desired characteristics is a promising strategy to develop plants with improved characters. In the present study, we isolated a group of somaclonal variants of banana cv. Rasthali which showed efficient resistance towards Foc race 1 infection in repeated bioassays. cDNA-RAPD methodology using 96 decamer primers was used to characterize these somaclonal variants. Among the four differentially amplified bands obtained, one mapping to the coding region of a lipoxygenase gene was confirmed to be down regulated in the somaclones as compared to controls by real-time quantitative RT-PCR. Our results correlated well with earlier studies with lipoxygenase mutants in maize wherein reduced expression of lipoxygenase led to enhanced resistance towards Fusarium infection.

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References

  1. FAOSTAT (2012) http://www.faostat3.fao.org/faostat-gateway/go/to/download/Q/QC/E. Accessed 25 August 2014

  2. Jones DR (2000) Diseases of banana, abacá and enset. CABI Publishing, Wallingford

    Google Scholar 

  3. Ploetz RC (2006) Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology 96:653–656

    Article  PubMed  Google Scholar 

  4. Bakry F, Carreel F, Jenny C, Horry JP (2009) Genetic improvement of banana. In: Jain SM, Priyadarshan PM (eds) Breeding plantation tree crops: tropical species. Springer Science & Business Media, Berlin, pp 3–51

    Chapter  Google Scholar 

  5. Hwang SC, Ko WH (2004) Cavendish banana cultivars resistant to Fusarium wilt acquired through somaclonal variation in Taiwan. Plant Dis 88:580–588

    Article  Google Scholar 

  6. Leva AR, Petruccelli R, Rinaldi LMR (2012) Recent advances in plant in vitro culture. Somaclonal variation in tissue culture: a case study with olive, Chap 7. doi:10.5772/50367

  7. Roux N, Baurens FC, Doležel J, Hřibová E, Heslop-Harrison P, Town C, Sasaki T, Matsumoto T, Aert R, Remy S, Souza M, Lagoda P (2008) Genomics of banana and plantain (Musa spp.), major staple crops in the tropics. In: Moore PH, Ming R (eds) Plant genetics and genomics: crops and models genomics of tropical crop plants. Springer, New York, pp 83–111

    Chapter  Google Scholar 

  8. Reuveni O, Israeli Y (1990) Measures to reduce somaclonal variation in vitro propagated bananas. Acta Hortic 275:307–313

    Google Scholar 

  9. Bairu MW, Fennell CW, van Staden J (2006) The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Sci Hortic 108:347–351

    Article  CAS  Google Scholar 

  10. Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance in lettuce. Theor Appl Genet 85:985–993

    Article  CAS  PubMed  Google Scholar 

  11. Mayer MS, Tullu A, Simon CJ, Kumar J, Kaiser WJ, Kraft JM, Muehlbauer FJ (1997) Development of a DNA marker for Fusarium wilt resistance in chickpea. Crop Sci 37:1625–1629

    Article  CAS  Google Scholar 

  12. Miklas PN, Stavely JR, Kelly JD (1993) Identification and potential use of a molecular marker for rust resistance in common bean. Theor Appl Genet 85:745–749

    CAS  PubMed  Google Scholar 

  13. Yoshida KT, Naito S, Takeda G (1994) cDNA cloning of regeneration-specific genes in rice by differential screening of randomly amplified cDNAs using RAPD primers. Plant Cell Physiol 35:1003–1009

    CAS  PubMed  Google Scholar 

  14. Ganapathi TR, Higgs NS, Balint-Kurti PJ, Arntzen CJ, May GD, Van Eck JM (2001) Agrobacterium-mediated transformation of the embryogenic cell suspensions of the banana cultivar Rasthali (AAB). Plant Cell Rep 20:157–162

    Article  CAS  Google Scholar 

  15. Cote FX, Domergue R, Monmarson S, Schwendiman J, Teisson C, Escalant JV (1996) Embryogenic cell suspensions from the male flower of Musa AAA cv. Grand nain. Physiol Plant 97:285–290

    Article  CAS  Google Scholar 

  16. Ghag SB, Shekhawat UKS, Ganapathi TR (2012) Petunia floral defensins with unique prodomains as novel candidates for development of Fusarium wilt resistance in transgenic banana plants. PLoS One 7(6):e39557. doi:10.1371/journal.pone.0039557

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Ghag SB, Shekhawat UKS, Ganapathi TR (2014) Host induced post-transcriptional hairpin RNA-mediated gene silencing of vital fungal genes confers efficient resistance against Fusarium wilt in banana. Plant Biotechnol J. doi:10.1111/pbi.12158

    PubMed  Google Scholar 

  18. Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30:e36

    Article  PubMed Central  PubMed  Google Scholar 

  19. Aurore G, Parfait B, Fahrasmane L (2009) Bananas-raw materials for making processed food products. Trends Food Sci Technol 20:78–91

    Article  CAS  Google Scholar 

  20. Thangavelu R, Sundaraju P, Sathiamoorthy S, Raguchander T, Velazhahan R, Nakkeeran S, Palaniswami A (2001) Status of Fusarium wilt of banana in India. In: Molina AB, Nikmasdek NH, Liew KW (eds) Banana Fusarium wilt management towards sustainable cultivation. INIBAP-ASPNET, Los Banos, Laguna, Philippines, pp 58–63

    Google Scholar 

  21. Aquino AP, Bandoles GG, Lim VAA (2013) R&D and policy directions for effective control of Fusarium wilt disease of Cavendish banana in the Asia-Pacific region. http://www.ap.fftc.agnet.org/ap_db.php?id=163. Accessed 24 July 2014

  22. LoSchiavo F, Pitto L, Giuliano G, Torti G, Nuti-Ronchi V, Marazziti D, Vergara R, Orselli S, Terzi M (1989) DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. Theor Appl Genet 77:325–331

    Article  CAS  PubMed  Google Scholar 

  23. Gao X, Shim WB, Göbel C, Kunze S, Feussner I, Meeley R, Balint-Kurti P, Kolomiets M (2007) Disruption of a maize 9-lipoxygenase results in increased resistance to fungal pathogens and reduced levels of contamination with the mycotoxin fumonisin. Mol Plant Microbe Interact 20:922–933

    Article  CAS  PubMed  Google Scholar 

  24. Gao XQ, Brodhagen M, Isakeit T, Brown SH, Göbel C, Betran J, Feussner I, Keller NP, Kolomiets MV (2009) Inactivation of the lipoxygenase ZmLOX3 increases susceptibility of maize to Aspergillus spp. Mol Plant Microb Interact 22:222–231

    Article  Google Scholar 

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Acknowledgments

Authors would like to thank Dr. S. P. Kale, Head, Nuclear Agriculture and Biotechnology Division, BARC for his support and encouragement.

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Authors and Affiliations

  1. Plant Cell Culture Technology Section, Nuclear Agriculture & Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India

    Siddhesh B. Ghag, Upendra K. S. Shekhawat & Thumballi R. Ganapathi

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  1. Siddhesh B. Ghag
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  2. Upendra K. S. Shekhawat
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  3. Thumballi R. Ganapathi
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Correspondence to Thumballi R. Ganapathi.

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Ghag, S.B., Shekhawat, U.K.S. & Ganapathi, T.R. Characterization of Fusarium wilt resistant somaclonal variants of banana cv. Rasthali by cDNA-RAPD. Mol Biol Rep 41, 7929–7935 (2014). https://doi.org/10.1007/s11033-014-3687-3

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