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Polymorphism and nucleotide sequencing of BMPR1B gene in prolific Assam hill goat

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Abstract

Assam hill goat (Capra hircus) is a prolific local goat in India. bone morphogenetic protein receptor (BMPR1B) gene was studied as a candidate gene for the prolificacy of goats. The objective of the present study was to detect the incidence of mutation in the exonic region of BMPR1B gene of Assam hill goat. Total 90 blood samples were collected randomly from different parts of Assam and genomic DNA were extracted using phenol–chloroform method. The quantity and quality of extracted DNA was examined by spectrophotometry and gel electrophoresis, respectively. PCR amplicon showed a product of 140 bp fragment of BMPR1B gene. The purified product upon digestion with AvaII showed monomorphic banding pattern and revealed wild type alleles with AA genotype. Nucleotide sequencing showed one new mutation 773 (G→C) which is found to be unique in Assam hill goat. Construction of tree at nucleotide level generates from the present experiment lies in common cluster which differs from the other breeds of goat. The analysis of polymorphism for BMPR1B in Assam hill goat indicates that the genetic factor responsible for prolificacy or multiple kidding rates is not related to the reported mutated alleles of BMPR1B gene. Therefore, attempts to be made to detect other SNPs for BMPR1B gene or otherwise effort should be made towards other fecundity gene which might be responsible for the prolificacy of Assam hill goat.

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Acknowledgments

The authors are thankful to the Dean, College of Veterinary Science, Guwahati. The authors also thankful to Department of Biotechnology, Govt. of India for providing grant to carry out this research work.

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Authors and Affiliations

  1. Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781022, India

    Rupam Dutta, Probodh Borah & Deep Prakash Saikia

  2. Department of Animal Genetics and Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781022, India

    Subimal Laskar, Bula Das & Galib Zaman

  3. Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781022, India

    Dhrubajyoti Kalita

  4. Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781022, India

    Nagendra Nath Barman

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  1. Rupam Dutta
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  2. Subimal Laskar
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Correspondence to Rupam Dutta.

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Dutta, R., Laskar, S., Borah, P. et al. Polymorphism and nucleotide sequencing of BMPR1B gene in prolific Assam hill goat. Mol Biol Rep 41, 3677–3681 (2014). https://doi.org/10.1007/s11033-014-3232-4

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  • DOI: https://doi.org/10.1007/s11033-014-3232-4

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