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Chlamydia trachomatis infection and human papillomavirus in women with cervical neoplasia in Pernambuco-Brazil

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Abstract

Chlamydia trachomatis (CT) is the most common bacterial cause of sexually transmitted disease. High-risk human papillomavirus (HR-HPV) is considered the main etiological agent for cervical neoplasia. Evidences showed that the presence of co-infection of CT and HR-HPV plays a central role in the etiology of cervical intraepithelial neoplasia (CIN) and cervical cancer. The goals of this study were: evaluate the human papillomavirus (HPV) and CT prevalence among Brazilian women with abnormal cytology and provide the effect of this association on the severity of cervical neoplasia. The population of this study was composed by 142 women with incident histological incidence of CIN grades I, II, III or cervical cancer from Recife, Northeast of Brazil. The polymerase chain reaction method on a cervical brush specimen was used to detect both agents and the automatic sequencing method was used for HPV genotyping assay. The prevalence of HPV and CT was 100 and 24.65 %, respectively. Thirteen types of HPV were detected; HPV 16, 18, 31 and 33 were the most common. The most prevalent HPV types were HPV 16 and 18. A significant association between CT positive and HPV 16 infection was found (p < 0.0106; OR = 5.31; 95 % IC 1.59–17.67). In the study population, there was diversity of HPV infections, with high-risk types being the most common. Also, the data collected suggest that CT infection may play an important role in the natural history of HPV infection.

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Abbreviations

CT:

Chlamydia trachomatis

STDs:

Sexually transmitted diseases

PID:

Pelvic inflammatory disease

HPV:

Human papillomavirus

LR-HPV:

Low-risk HPV

HR-HPV:

High-risk HPV

CIN:

Cervical intraepithelial neoplasia

ICC:

Invasive cervical cancer

PCR:

Polymerase chain reaction

DNA:

Deoxyribonucleic acid

EDTA:

Ethylenediamine tetraacetic acid

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Acknowledgments

We thank the patients and their families, whose collaboration and understanding have made this work possible. This study was supported by the Brazilian funding agency FACEPE (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco). We are also grateful for the facilities of the Laboratório de Genética, Bioquímica e Sequenciamento de DNA Profa Tania Falcão of Universidade Federal Rural de Pernambuco, where the experiments were performed.

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Correspondence to Mayara Costa Mansur Tavares.

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Tavares, M.C.M., de Macêdo, J.L., de Lima Júnior, S.F. et al. Chlamydia trachomatis infection and human papillomavirus in women with cervical neoplasia in Pernambuco-Brazil. Mol Biol Rep 41, 865–874 (2014). https://doi.org/10.1007/s11033-013-2927-2

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