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Cloning and characterization of dehydrin gene from Ammopiptanthus mongolicus

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Abstract

Based on the sequence of an expressed sequence tag, the full-length cDNA of 1,008 nucleotides was cloned from Ammopiptanthus mongolicus by rapid amplification of cDNA ends. It was designated as AmDHN, encoding a protein of 183 amino acids. The calculated molecular weight of the AmDHN protein is 18.4 k Da, and theoretical isoelectric point is 5.78. The AmDHN localized in nucleus. Under normal growth conditions, differential expression of AmDHN exhibited that the expression was the highest in seeds and the lowest in flowers. AmDHN could be induced by NaCl, PEG6000, ABA and drought treatments. Salt and drought resistances of transgenic plants with overexpression of AmDHN are improved. Taken together, these results demonstrated that AmDHN could regulate the expression of abiotic-responsive genes and plays important roles in modulating the tolerance of plants to abiotic stresses.

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Abbreviations

ABA:

Abscisic acid

CDS:

Coding domain sequence

GFP:

Green fluorescent protein

RACE:

Rapid amplification of cDNA ends

RT-PCR:

Reverse transcriptase polymerase chain reaction

SSH:

Suppression subtraction hybridization

MW:

Molecular weight

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Acknowledgments

We thank Dr. Yuehui Chao for advices and helps. This work is supported by the Scientific and technological innovation projects in Inner Mongolia (2011-CXJJM01) and Industrial system of national grass (CARS-35).

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Authors and Affiliations

  1. Inner Mongolia Academy of Agricultural and Animal Husbandry Science, Inner Mongolia, 0100031, China

    Jie Sun, Lizhen Nie, Guoqin Sun, Jiufeng Guo & Yongzhi Liu

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  1. Jie Sun
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  3. Guoqin Sun
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Correspondence to Yongzhi Liu.

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Sun, J., Nie, L., Sun, G. et al. Cloning and characterization of dehydrin gene from Ammopiptanthus mongolicus . Mol Biol Rep 40, 2281–2291 (2013). https://doi.org/10.1007/s11033-012-2291-7

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