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Assay and characterization of an osmolarity inducible promoter newly isolated from Bacillus subtilis

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Abstract

An osmolarity-sensitive promoter fragment, P23423, isolated from Bacillus subtilis was characterized. The expression of β-galactosidase (β-Gal) driven by P23423 was regulated by osmolarity both in Escherichia coli and B. subtilis. The classical conserved region of this prokaryotic promoter was found within the sequence of the cloned fragment, and the putative promoter was identified as the control element of RNA not coding for protein (a RNA molecule that is not translated into a protein). The efficiency and benefit of this promoter was further demonstrated via osmolarity-induced expression of three other heterologous proteins in E. coli. Thus, this approach provided a simple and inexpensive inducible promoter element for the expression of cloned genes.

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Acknowledgment

The authors gratefully acknowledge the Bacillus Genetic Stock Centre of Ohio State University for generously providing the study materials. And this study was supported by the grants of NSFC (30872033, 31170609).

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Authors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, 712100, People’s Republic of China

    Wei-Wei Zhang, Qiu-Rong Gao & Dun Wang

  2. College of Animal Sciences, Yangling, 712100, People’s Republic of China

    Ming-Ming Yang & Hui Liu

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  1. Wei-Wei Zhang
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  2. Qiu-Rong Gao
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  4. Hui Liu
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  5. Dun Wang
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Correspondence to Dun Wang.

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Zhang, WW., Gao, QR., Yang, MM. et al. Assay and characterization of an osmolarity inducible promoter newly isolated from Bacillus subtilis . Mol Biol Rep 39, 7347–7353 (2012). https://doi.org/10.1007/s11033-012-1566-3

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  • DOI: https://doi.org/10.1007/s11033-012-1566-3

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