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Polymorphisms of caprine POU1F1 gene and their association with litter size in Jining Grey goats

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Abstract

Seven pairs of primers were designed to amplify 5′ promoter region, six exons and partial introns and to detect the polymorphisms of POU1F1 gene in five goat breeds with different prolificacy. The results showed that six mutations were identified in caprine POU1F1 gene including C256T in exon 3, C53T and T123G in intron 3, and G682T (A228S), T723G and C837T in exon 6. The former four mutations were novel SNPs in goat POU1F1 gene. The 53 and 123 loci were in complete linkage disequilibrium in five caprine breeds. Regarding the 256 locus, the Jining Grey goat does with genotype CT had 0.66 kids more than those with genotype CC (P < 0.05), while does with genotype GT had 0.63 (P < 0.05) kids more than those with genotype GG at the 682 locus. The present study preliminarily showed an association between allele T at the 256 and 682 loci of POU1F1 gene and high litter size in Jining Grey goats. Totally 16 haplotypes and 50 genotypes were identified at the above six loci in POU1F1 gene of five goat breeds. Three common haplotypes (hap2, hap3 and hap4) were identified in five goat breeds joined. Four specific haplotypes (hap7, hap9, hap11 and hap13) were detected in Jining Grey goats. The predominant haplotype was hap1 (35.29% and 48.25%) in both Jining Grey and Guizhou White goats, while hap4 (50%) in Boer goats, and hap2 (42.86% and 38.75%) in both Wendeng Dairy and Liaoning Cashmere goats. The most frequent genotypes at six loci in the above five goat breeds were hap1/hap2 (14.38%) and hap1/hap4 (14.38%), hap1/hap2 (38.60%), hap4/hap4 (40.91%), hap2/hap4 (26.53%), hap2/hap5 (20.00%), respectively. The Jining Grey goat does with nine genotypes analyzed of POU1F1 gene showed no obvious difference in litter size.

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Acknowledgements

This research was supported by National Key Technology Research and Development (R&D) Program of China (2008BADB2B01), National Natural Science Foundation of China (30871773), National High Technology R&D Program of China (2006AA10Z139), National Key Basic R&D Program of China (2006CB102105), the earmarked fund for China Agriculture Research System (CARS-39).

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Authors and Affiliations

  1. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    T. Feng, M. X. Chu, G. L. Cao, Q. Q. Tang, R. Di & L. Fang

  2. State Key Laboratory of Agricultural Biotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China

    N. Li

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  1. T. Feng
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  2. M. X. Chu
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  3. G. L. Cao
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  6. L. Fang
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Correspondence to M. X. Chu.

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Feng, T., Chu, M.X., Cao, G.L. et al. Polymorphisms of caprine POU1F1 gene and their association with litter size in Jining Grey goats. Mol Biol Rep 39, 4029–4038 (2012). https://doi.org/10.1007/s11033-011-1184-5

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