Skip to main content
SpringerLink
Log in

Polymorphisms of STAT5A gene and their association with milk production traits in Holstein cows

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

The STAT5A gene was studied as a candidate gene for five milk production traits (milk yield at 305 days, protein percentage, fat percentage, lactose percentage and dry matter percentage) in Holstein cows. According to the sequence of bovine STAT5A gene, two pairs of primers (P1 and P2) were designed to detect polymorphisms of STAT5A gene in 401 Holstein cows by PCR-RFLP and PCR-SSCP. The results showed that the products amplified by primers P1 and P2 displayed polymorphisms. For P1, three genotypes (AA, AG, and GG) were detected, and the frequency of AA/AG/GG was 0.252/0.486/0.262, respectively. Sequence analysis revealed a single nucleotide substitution A–G at 14217 bp (GenBank NC_007317) of bovine STAT5A gene while compared GG genotype with AA genotype. The differences of the least squares means for the four milk production traits (milk yield at 305 days, fat percentage, lactose percentage and dry matter percentage) between AA, AG and GG were not significant (P > 0.05). Least squares mean of protein percentage for AG or GG was significantly higher than that for AA (P < 0.05); the difference of the least squares mean for protein percentage was not significant between AG and GG (P > 0.05). For P2, three genotypes (CC, CT, and TT) were detected in Holstein cows, and the frequency of CC/CT/TT was 0.751/0.234/0.015, respectively. Sequencing revealed an insertion CCT at 17266 (NC_007317) of bovine STAT5A gene while compared CC genotype with TT genotype. The differences of the least squares means for the three milk production traits (protein percentage, lactose percentage and dry matter percentage) between CC, CT and TT were not significant (P > 0.05). Least squares mean of milk yield at 305 days for TT or CT was significantly higher than that for CC (P < 0.05); the difference of the least squares mean for milk yield at 305 days was not significant between TT and CT (P > 0.05). Least squares mean of fat percentage for CC or CT was significantly higher than that for TT (P < 0.05); the difference of the least squares mean for fat percentage was not significant between CC and CT (P > 0.05). The results preliminarily indicated that allele G of A14217G polymorphic site of STAT5A gene is a potential DNA marker for improving protein percentage in dairy cattle, 17266indelCCT polymorphic site of STAT5A gene is a potential DNA marker for improving milk yield at 305 days and fat percentage in dairy cattle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Liu X, Robinson GW, Goiouilleux F, Groner B, Hennighausen L (1995) Cloning and expression of STAT5 and an additional homologue (STAT5B) involved in prolactin signal transduction in mouse mammary tissue. Proc Natl Acad Sci USA 92(19):8831–8835

    Article  PubMed  CAS  Google Scholar 

  2. Darnell JE Jr (1997) STATs and gene regulation. Science 277(5332):1630–1635

    Article  PubMed  CAS  Google Scholar 

  3. Liu X, Robinson GW, Hennighausen L (1996) Activation of STAT5 and STAT5B by tyrosine phosphorylation is tightly linked to mammary gland differentiation. Mol Endocrinol 10(12):1496–1506

    Article  PubMed  CAS  Google Scholar 

  4. Liu X, Robinson GW, Wagner KU, Garrett L, Wynshaw-Boris A, Hennighausen L (1997) STAT5A is mandatory for adult mammary gland development and lactogenesis. Genes Dev 11(2):179–186

    Article  PubMed  CAS  Google Scholar 

  5. Udy GB, Towers RP, Snell RG, Wilkins RJ, Park SH, Ram PA, Waxman DJ, Davey HW (1997) Requirement of STAT5B for sexual dimorphism of body growth rates and liver gene expression. Proc Natl Acad Sci USA 94(14):7239–7244

    Article  PubMed  CAS  Google Scholar 

  6. Bole-Feysot C, Goffin V, Edery M, Binart N, Kelly PA (1998) Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev 19(3):225–268

    Article  PubMed  CAS  Google Scholar 

  7. Wang H, Han YP, Liu ZL (2000) Research advances of STATs. Foreign Med Sci Sect Pathophysiol Clin Med 20(5):354–357

    Google Scholar 

  8. Rowland JE, Lichanska AM, Kerr LM, White M, d’Aniello EM, Maher SL, Brown R, Teasdale RD, Noakes PG, Waters MJ (2005) In vivo analysis of growth hormone receptor signaling domains and their associated transcripts. Mol Cell Biol 25(1):66–77

    Article  PubMed  CAS  Google Scholar 

  9. Grebien F, Kerenyi MA, Kovacic B, Kolbe T, Becker V, Dolznig H, Pfeffer K, Klingmüller U, Müller M, Beug H, Müllner EW, Moriggl R (2008) STAT5 activation enables erythropoiesis in the absence of EpoR and Jak2. Blood 111(9):4511–4522

    Article  PubMed  CAS  Google Scholar 

  10. Lewis RS, Ward AC (2008) STAT5 as a diagnostic marker for leukemia. Expert Rev Mol Diagn 8(1):73–82

    Article  PubMed  CAS  Google Scholar 

  11. Santos SJ, Haslam SZ, Conrad SE (2008) Estrogen and progesterone are critical regulators of STAT5A expression in the mouse mammary gland. Endocrinology 149(1):329–338

    Article  PubMed  CAS  Google Scholar 

  12. Byts N, Samoylenko A, Fasshauer T, Ivanisevic M, Hennighausen L, Ehrenreich H, Sirén AL (2008) Essential role for STAT5 in the neurotrophic but not in the neuroprotective effect of erythropoietin. Cell Death Differ 15(4):783–792

    Article  PubMed  CAS  Google Scholar 

  13. Molenaar A, Wheeler TT, McCracken JY, Seyfert HM (2000) The STAT3-encoding gene resides within the 40 kbp gap between the STAT5A- and STAT5B-encoding genes in cattle. Anim Genet 31(5):339–340

    Article  PubMed  CAS  Google Scholar 

  14. Seyfert HM, Pitra C, Meyer L, Brunner RM, Wheeler TT, Molenaar A, McCracken JY, Herrmann J, Thiesen HJ, Schwerin M (2000) Molecular characterization of STAT5A- and STAT5B-encoding genes reveals extended intragenic sequence homogeneity in cattle and mouse and different degrees of divergent evolution of various domains. J Mol Evol 50(6):550–561

    PubMed  CAS  Google Scholar 

  15. Brym P, Kamiński S, Ruść A (2004) New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle. J Appl Genet 45(4):445–452

    PubMed  Google Scholar 

  16. He F, Sun Dong-xiao, Yu Ying, Wang Ya-chun, Zhang Yuan (2007) SNPs detection of STAT5A gene and association with milk production traits in Holstein cattle. Acta Veterinaria et Zootechnica Sinica 38(4):326–331

    CAS  Google Scholar 

  17. Antoniou E, Hirst BJ, Grosz M, Skidmore CJ (1999) A single strand conformational polymorphism in the bovine gene STAT5A. Anim Genet 30(3):232

    Article  PubMed  CAS  Google Scholar 

  18. Flisikowski K, Szymanowska M, Zwierzchowski L (2003) The DNA-binding capacity of genetic variants of the bovine STAT5A transcription factor. Cell Mol Biol Lett 8(3):831–840

    PubMed  CAS  Google Scholar 

  19. Khatib H, Monson RL, Schutzkus V, Kohl DM, Rosa GJ, Rutledge JJ (2008) Mutations in the STAT5A gene are associated with embryonic survival and milk composition in cattle. J Dairy Sci 91(2):784–793

    Article  PubMed  CAS  Google Scholar 

  20. Bao B, Fang XT, Chen H, Zhang RF, Yan LJ, Zhang HJ (2008) Polymorphisms of STAT5A gene and its association with milk performance traits in Chinese Holstein cattle. Scientia Agricultura Sinica 41(6):1872–1878

    CAS  Google Scholar 

  21. Selvaggi M, Dario C, Normanno G, Celano GV, Dario M (2009) Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle. J Dairy Res 29:1–5

    Google Scholar 

  22. Mao J, Molenaar AJ, Wheeler TT, Seyfert HM (2002) STAT5 binding contributes to lactational stimulation of promoter III expressing the bovine acetyl-CoA carboxylase alpha-encoding gene in the mammary gland. J Mol Endocrinol 29(1):73–88

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by National Key Technology Research and Development Program of China (No. 2006BAD04A10) and Beijing Natural Science Foundation of China (No. 6022015).

Author information

Authors and Affiliations

  1. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    X. He, M. X. Chu, J. N. He, T. Feng, R. Di, G. L. Cao & L. Fang

  2. Beijing Dairy Cattle Centre, Beijing, 100192, People’s Republic of China

    L. Qiao

  3. Bioengineering College, Chongqing University, Chongqing, 400030, People’s Republic of China

    P. Q. Wang

  4. Hebei Animal Science and Veterinary Medicine Institute, Baoding, 071000, People’s Republic of China

    Y. F. An

Authors
  1. X. He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. X. Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Qiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. N. He
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Q. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. Di
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. G. L. Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. L. Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Y. F. An
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. X. Chu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

He, X., Chu, M.X., Qiao, L. et al. Polymorphisms of STAT5A gene and their association with milk production traits in Holstein cows. Mol Biol Rep 39, 2901–2907 (2012). https://doi.org/10.1007/s11033-011-1051-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-011-1051-4

Keywords

Search

Navigation