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Molecular cloning, tissue distribution and ontogenetic expression of Xiang pig Chemerin and its involvement in regulating energy metabolism through Akt and ERK1/2 signaling pathways

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Abstract

Chemerin, as a new member of adipokines family, is highly expressed in adipose tissue in rodent and its expression increases with obesity. Moreover, chemerin has been reported to have significant relationship with metabolic syndrome and insulin sensitivity. Here, the gene encoding chemerin from Xiang pig was cloned. The open reading frame of this cDNA encodes 163 deduced amino acid residues. The putative protein has a N-terminal signaling peptide and a nuclear localization signal profile which are highly conserved among the vertebrate orthologs. Both chemerin and chemerinR are highly expressed in lung, kidney and small intestine in adult Xiang pig. Besides these tissues, chemerin is abundant in liver and backfat, and chemerinR is abundant in spleen and skeletal muscle. We also investigated the age-dependent expression of chemerin in suckling Xiang piglets in various tissues, which showed an interaction between age and segments in abundance of chemerin and chemerinR from day 1 to day 21. For chemerinR, it was abundant in skeletal muscle of both adult and fetal Xiang pig. Further, we treated differentiated C2C12 cells with chemerin. The result showed that chemerin regulated energy metabolism partly through Akt and ERK1/2 signaling pathway. Taken together, our findings provide basic molecular information for the deeper investigation on the function of chemerin.

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Abbreviations

ERK1/2:

Extracellular-signal regulated kinase-1/2

mTOR:

Mammalian target of rapamycin

HSL:

Hormone-sensitive lipase

ACC:

Acetyl CoA carboxylase

FBS:

Fetal bovine serum

PFK:

Phosphofructokinase

PK:

Pyruvate kinase

FABP:

Fatty acid-binding protein

ACO:

Acyl CoA oxidase

CPT:

Carnitine palmitoyl transferase

ISR:

Insulin receptor

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Acknowledgments

We thank Prof. Andrew Adegboyega Fatufe for critical reading of this manuscript. This study was jointly supported by National Nature Science Foundation of China (31001015, 31001016 and 31072042, 30901040, 30901041, 30928018, 30828025), funds from the Chinese Academy of Sciences and Knowledge Innovation Project (Kscx2-Yw-N-051), Research Supported by the CAS/SAFEA International Partnership Program for Creative Research Teams (20100491005-8 and KZCX2-YW-T07), Changsha City Key Project (K1003238-61), Guangdong Province Key Project (2006B20330005, GZCQC0802FG06, 2009B091300043, 2009B030802021), State Key Laboratory of Animal Nutrition (2004DA125184F1113).

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Authors and Affiliations

  1. Research Center for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Hunan, 410125, China

    Huansheng Yang, Huansheng Yang, Fengna Li, Xiangfeng Kong, Xiaoxue Yuan, Guoqi Lian, Meimei Geng, Tiejun Li & Yulong Yin

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China

    Huansheng Yang, Xiaoxue Yuan & Guoqi Lian

  3. State Key Laboratory of Animal Nutrition, Beijing, 100193, China

    Jingdong Yin

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  1. Huansheng Yang
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Correspondence to Jingdong Yin or Yulong Yin.

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Yang, H., Li, F., Kong, X. et al. Molecular cloning, tissue distribution and ontogenetic expression of Xiang pig Chemerin and its involvement in regulating energy metabolism through Akt and ERK1/2 signaling pathways. Mol Biol Rep 39, 1887–1894 (2012). https://doi.org/10.1007/s11033-011-0934-8

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  • DOI: https://doi.org/10.1007/s11033-011-0934-8

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