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Preparation, characterization and transfection efficacy of chitosan nanoparticles containing the intestinal trefoil factor gene

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Abstract

Intestinal trefoil factor (ITF) is a novel polypeptide with potential pharmacological value for the prevention and healing of tissue injury; however, poor production capacity limits its clinical application. Chitosan, as a non-viral vehicle, has been successfully used in gene delivery for its intrinsic characteristics. In this context, we prepared chitosan nanoparticles enwrapping ITF cDNA and investigated its size, zeta potential, stability, release profiles, loading efficiency and loading capacity. Gene transfer capability was assessed in HEK293 cells. The data revealed that the chitosan/DNA nanoparticles were successfully prepared with sizes less than 500 nm and positive zeta potentials. The nanoparticles could protect DNA from nuclease degradation, and release profiles of DNA were dependent on N/P ratios. In addition, transfection efficiency of chitosan/DNA nanoparticles was equivalent to Lipofectamine (TM). Collectively, the results suggest that chitosan/DNA nanoparticles could be a promising method for ITF gene therapy.

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Acknowledgments

This study was supported by the Scientific Research Funds of Nanjing Military Command (09Z007), State Key Lab Funding in China (SKLZZ200905), and Key Project on Advanced Clinical Technology for Military Hospital (2010gxjs068).

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Authors and Affiliations

  1. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, The Third Military Medical University, Chongqing, 400038, China

    Yong Sun, Shuai Zhang, Xi Peng, Zhenyu Gong, Xiaolu Li, Zhiqiang Yuan, Ying Li, Dawei Zhang & Yizhi Peng

  2. Department of Burn Surgery, Plastic Surgery Center, No. 97 Hospital of PLA, Xuzhou, 221004, People’s Republic of China

    Yong Sun

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  1. Yong Sun
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  2. Shuai Zhang
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  3. Xi Peng
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  4. Zhenyu Gong
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  5. Xiaolu Li
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  6. Zhiqiang Yuan
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  7. Ying Li
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  8. Dawei Zhang
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  9. Yizhi Peng
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Corresponding author

Correspondence to Yizhi Peng.

Additional information

Yong Sun and Shuai Zhang contributed equally to this work.

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Sun, Y., Zhang, S., Peng, X. et al. Preparation, characterization and transfection efficacy of chitosan nanoparticles containing the intestinal trefoil factor gene. Mol Biol Rep 39, 945–952 (2012). https://doi.org/10.1007/s11033-011-0820-4

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  • DOI: https://doi.org/10.1007/s11033-011-0820-4

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