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Age-related expression profile of the SLC27A1 gene in chicken tissues

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Abstract

The solute carrier family 27 (SLC27, also known as fatty acid transport proteins [FATPs]) plays important biological roles in cells. However, there is no report about the expression profile of SLC27 member in chicken. In this study, we quantified the expression of SLC27A1 (FATP1) mRNA in a mountainous black-boned chicken breed (MB) and a commercial meat type chicken breed (S01), to discern the tissue and age-related specific expression pattern and their potential involvement in fat deposition and muscle fatty acid metabolism. Real-time quantitative PCR assays were developed for accurate measurement of SLC27A1 mRNA levels in different tissues from chicken with different ages (0–12 weeks). Expression of SLC27A1 mRNA was detected in all tissues examined. There was a significantly age-related change of the SLC27A1 mRNAs in heart, breast muscle (BMW), leg muscle (LMW), liver, and abdominal fat (AF) tissues (P < 0.05). The breast muscle and leg muscle tissues had the highest expression of SLC27A1 mRNA than the other tissues from the same individual at 0, 2 and 4 weeks. The overall SLC27A1 mRNA level exhibited a “rise-decline” developmental change in all tissues except for breast muscle, subcutaneous fat, and brain. The S01 chicken had a higher expression of the SLC27A1 mRNA in breast muscle, subcutaneous fat, and heart tissues than the MB chicken. Our results showed that the expression of SLC27A1 mRNA in chicken tissues exhibits specific developmental changes and age-related patterns.

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Acknowledgments

We thank Xiaosong Jiang and Huarui Du for sample collecting and Yi Luo for technical support. This study was supported by the Ministry of Agriculture of China (No. 2009ZX08009-159B), the Program for New Century Excellent Talents in University (NCET-10-0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), and Yunnan Province (2009CI119).

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Authors and Affiliations

  1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Yan Wang, Qing Zhu, Xiao-Ling Zhao & Yi-Ping Liu

  2. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, 650223, China

    Yong-Gang Yao

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  1. Yan Wang
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  2. Qing Zhu
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  3. Xiao-Ling Zhao
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  4. Yong-Gang Yao
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  5. Yi-Ping Liu
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Correspondence to Yi-Ping Liu.

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Wang, Y., Zhu, Q., Zhao, XL. et al. Age-related expression profile of the SLC27A1 gene in chicken tissues. Mol Biol Rep 38, 5139–5145 (2011). https://doi.org/10.1007/s11033-010-0663-4

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  • DOI: https://doi.org/10.1007/s11033-010-0663-4

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