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Gα13 is closely related to hematopoiesis in zebrafish

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Abstract

Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) function as signal transducers and control many different physiologic processes. G proteins can be grouped into four families: Gs, Gi, Gq and G12. Gα13 belongs to the G12 family. In zebrafish, there are two isoforms of Gα13: Gα13a and Gα13b. We show here that knockdown of Gα13b in zebrafish results in hematopoietic and angiogenic defects. The Gα13b morphants don’t show complete loss of expression of gata1, pu.1 or flk until 35 hpf suggests that Gα13b is closely related to the development of hematopoietic cells. Further studies reveal that blood cells and vascular endothelial cells have undergone apoptosis through a p53-dependent pathway in Gα13b-depleted embryos. Injection of p53 morpholino could partially rescue the phenotype of Gα13b morphants. These data possibly demonstrate a new role for Gα13 in cell survival.

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Acknowledgments

We thank Dr. Nobuyuki Itoh at Kyoto University Graduate School of Pharmaceutical Sciences for plasmids of flk, gata1, pu.1, Dr. Shanye Gu at the Institute of Neuroscience, Chinese Academy of Sciences, for plasmids transferring, and Dr. Wei Zhang for imaging assistance. We also thank Dr. Margaret Ragni at Department of Medicine in University of Pittsburgh for critical reading of this manuscript and making significant improvement. The authors declare no conflict of interests. This study was supported by the Natural Sciences Foundation of China (No. 30510521 to P. Song and XY. Huang; No. 2004CB117401 to P. Song) and by ICGEB Research Grant (CRP/CHN02-01 to P. Song).

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Authors and Affiliations

  1. Laboratory of Molecular Genetics and Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Ding Ye, Qiwen Yang & Ping Song

  2. Laboratory of Biochemistry and Biophysics, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Zheng Tan

  3. Departments of Physiology, Cornell University Weill Medical College, New York, NY, 10065, USA

    Xinyun Huang

  4. Departments of Gynaecology and Obstetrics, South Central Hospital, Wuhan University, Wuhan, 430072, People’s Republic of China

    Jiarui Hu

  5. Departments of Immunology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, 44195, USA

    Shiguang Qian

  6. College of Fisheries, Huazhong Agricultural University, Wuhan, 430072, People’s Republic of China

    Yang Li

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  1. Ding Ye
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Correspondence to Ping Song.

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Ye, D., Yang, Q., Li, Y. et al. Gα13 is closely related to hematopoiesis in zebrafish. Mol Biol Rep 38, 2685–2694 (2011). https://doi.org/10.1007/s11033-010-0411-9

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  • DOI: https://doi.org/10.1007/s11033-010-0411-9

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