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A novel polymorphisms in intron 12 of the bovine calpastatin gene

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Abstract

Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases—μ-calpain and m-calpain, found in mammalian tissues. This proteolytic system plays a key role in the tenderization process that occurs during post-mortem storage of meat under refrigerated conditioning. Fragments of the bovine CAST gene including intron 12 were amplified and subjected to SSCP analysis. Four new SNPs were found within intron 12 of the CAST gene: a transition T/C at position 3893+155* A/G at position 3893+163, a transversion T/A at position 3893+223 and a substitution A/G at position 3893+428 (consensus sequence—GenBank AY834771). The genetic variants in the bovine CAST gene can be analyzed with RFLP method and was studied in 375 bulls of six breeds, including Hereford, Aberdeen-angus, Simmental, Charolaise, Limousine and Polish Black-and-White (BW; Fresian) breeds.

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Notes

  1. Position of the mutation in intron was marked with consideration of a position of the last suitable nucleotide exon in the mRNA sequence

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Acknowledgements

Authors would like to thank Mr Jarosław Połoszynowicz for the extraction of DNA. This study was funded by the State Committee for Scientific Research (KBN), grant No. 3 P06D01825 and the IGHZ project S.V.9.

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Authors and Affiliations

  1. Polish Academy of Sciences, Institute of Genetics and Animal Breeding, ul. Postępu 1, Jastrzębiec, 05-552, Wólka Kosowska, Poland

    Edyta Juszczuk-Kubiak, Joanna Wyszyńska-Koko, Krystyna Wicińska & Stanisław Rosochacki

  2. Chair of Sanitary Biology and Biotechnology, Białystok Technical University, Wiejska 45, 15-351, Białystok, Poland

    Stanisław Rosochacki

Authors
  1. Edyta Juszczuk-Kubiak
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  2. Joanna Wyszyńska-Koko
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  3. Krystyna Wicińska
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  4. Stanisław Rosochacki
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Correspondence to Edyta Juszczuk-Kubiak.

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Juszczuk-Kubiak, E., Wyszyńska-Koko, J., Wicińska, K. et al. A novel polymorphisms in intron 12 of the bovine calpastatin gene. Mol Biol Rep 35, 29–35 (2008). https://doi.org/10.1007/s11033-006-9048-0

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  • DOI: https://doi.org/10.1007/s11033-006-9048-0

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